PTHLH在宫颈癌的表达及其对HeLa细胞增殖、迁移及侵袭的影响

Expression of PTHLH in Cervical Cancer and Its Effects on Proliferation,Invasion and Migration of HeLa Cells

目的：检测甲状腺旁腺激素样激素（PTHLH）在宫颈癌组织中的表达,并用小干扰RNA（siRNA）沉默宫颈癌HeLa细胞中PTHLH基因的表达,观察PTHLH表达降低对宫颈癌细胞增殖、迁移及侵袭能力的影响,以初步探讨PTHLH在宫颈癌发生中的作用。方法：通过免疫组织化学法检测正常组织与宫颈癌组织中PTHLH蛋白的表达与定位。使用qRT-PCR和免疫荧光对宫颈癌HeLa细胞PTHLH-siRNA组及阴性对照组细胞中PTHLH的表达进行检测。使用CCK-8法检测沉默PTHLH后HeLa细胞与对照组的增殖情况。使用Transwell检测两组细胞迁移及侵袭能力的改变情况。结果：PTHLH在宫颈癌组织中高表达。siRNA能够有效沉默HeLa细胞中PTHLH的mRNA及蛋白的表达。沉默PTHLH表达能够明显抑制HeLa细胞的增殖及侵袭迁移能力。结论：宫颈癌组织中高表达的PTHLH可促进宫颈癌细胞的增殖、侵袭和迁移能力,沉默PTHLH基因可能是控制宫颈癌术后肿瘤复发的有效途径。

Objective：To investigate the expression of parathyroid hormone-like hormone（PTHLH）in the tissues of cervical carcinoma and the effects of PTHLH on HeLa cell proliferation,migration and invasion.Methods：Immunohistochemistry was applied to detect the expression of PTHLH in normal and cervical carcinoma tissues.HeLa cells were divided into two groups,PTHLH-siRNA group（siRNAs were applied to silence the expression of PTHLH in the PTHLH-siRNA group）and negative control group.Quantitative real-time PCR and immunofluorescence were applied to detect the mRNA and protein expression of PTHLH,respectively.CCK-8 and transwell assays were applied to detect the proliferation,migration and invasion ability of HeLa cells in the two groups.Results：PTHLH was highly expressed in the cervical carcinoma tissues while the expression was low in normal tissue.siRNAs effectively silenced the expression of PTHLH with down-regulated mRNA and protein.Furthermore,the proliferation,migration and invasion ability of PTHLH-siRNA group cells decreased significantly compared with the negative control group.Conclusion：PTHLH is highly expressed in the cervical cancer,and might be a key genefor drug target to improve the prognosis.