期刊文献+

可生物降解热敏性改性甲壳素水凝胶预防术后肠粘连效果的研究 被引量:3

Postoperative Adhesion Prevention with a Thermosensitive Injectable in situ Forming Carboxymethyl Chitin Hydrogel
原文传递
导出
摘要 目的:探究可生物降解热敏性改性甲壳素凝胶在动物肠壁-腹壁损伤模型中预防术后肠粘连的效果。方法:首先在体外细胞实验中探究了凝胶的生物相容性。然后建立了新西兰兔盲肠侧壁-腹壁损伤肠粘连模型,对比了改性甲壳素凝胶和商用几丁糖凝胶预防术后粘连的效果。观察凝胶在体内的降解情况,以及凝胶是否会对主要器官产生毒性损害。结果:溶血试验中凝胶与生理盐水溶血程度无显著差别(P〉0.05),表明凝胶不会溶血。细胞毒性试验发现,与凝胶直接接触肠上皮细胞的细胞活性与未接触凝胶的对照组无显著差异(P〉0.05),表明凝胶不会对细胞生长增殖造成影响。动物实验中,两种凝胶减少了粘连形成,并且改性甲壳素凝胶还减小形成粘连的面积。结论:可生物降解热敏性改性甲壳素凝胶生物相容性良好,能减少术后肠粘连的发生,是一种具有潜在临床应用价值的防粘连屏障材料。 Objective:To investigate whether the injectable biodegradable thermosensitive in situforming chemically modified carboxymethyl chitin hydrogel can be used to prevent postoperative adhesion formation.Methods:We first confirmed biocompatibility of the hydrogel in vitro by using hemolysis assay and cell viability assay,then a postoperative adhesion rabbit model of sidewall defect-bowel abrasion was established and the anti-adhesion effect of the hydrogel comparing with another commercially available chitin hydrogel was evaluated in the animal model in vivo.Results:In vitro experiments,the hydrogel did not cause significant hemolysis,nor did it affect cell growth and proliferation,confirming the excellent biocompatibility of the hydrogel.In the in vivo experiment of animal model,we found that the hydrogel degraded completely 3 weeks after applied,and comparing with the commercially available chitin hydrogel,our hydrogel tended to be more effective in preventing postoperative adhesion formation,and it also reduced the area of the formed adhesion.Conclusion:The injectable biodegradable thermosensitive in-situ formingchemically modified carboxymethyl chitin hydrogel is a promising barrier system for prevention of postoperative adhesion.
作者 姜军 刘庆 黄思澔 袁泽伟 向真贤 熊斌 JIANG Jun;LIU Qing;HUANG Sihao;YUAN Zewei;XIANG Zhenxian;XIONG Bin(Dept. of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071 , China)
出处 《武汉大学学报(医学版)》 CAS 2018年第4期605-609,共5页 Medical Journal of Wuhan University
关键词 术后粘连 热敏性 凝胶 动物模型 Postoperative Adhesion Thermosensitivity Hydrogel Animal Model
  • 相关文献

参考文献2

二级参考文献49

  • 1申洪.免疫组织化学染色定量方法研究(Ⅲ)[J].中国组织化学与细胞化学杂志,1995,4(1):89-92. 被引量:398
  • 2冷金花.妇科腹腔镜手术的热点问题[J].继续医学教育,2005,19(5):77-80. 被引量:9
  • 3潘仕荣,陈浩凡,莫家聪,王德娟,张未,郑欢玲.羧甲基壳聚糖用作防止术后粘连的研究[J].中国生物医学工程学报,2006,25(3):277-282. 被引量:20
  • 4Brüggmann D,Tchartchian G,Wallwiener M,et al.Intra-abdominal adhesions:definition,origin,significance in surgicalpractice,and treatment options.Dtsch Arztebl Int,20101,07(44):769-775.
  • 5Van Der Krabben AA,Dijkstra FR,Nieuwenhuijzen M,et al.Morbidity and mortality of inadvertent enterotomy duringadhesiotomy.Br J Surg,2000,87(4):467-471.
  • 6Soules MR,Dennis L,Bosarge A,et al.The privation ofpostoperative pelvic adhesions:an animal study comparing barriermethods with dextran 70.Am J Obstet Gynecol,1982,143(7):829-834.
  • 7Control and prevention of peritoneal adhesions in gynecologicsurgery.Practice Committee of the American Society for ReproductiveMedicine.Fertil Steril,2006,86(5 Suppl 1):S1-S5.
  • 8Ivarsson ML,Holmdahl L,Franzén G,et al.Cost of bowelobstruction resulting from adhesions.Eur J Surg 1997,163(9):679-684.
  • 9Kosaka H,Yoshimoto T,Yoshimoto T,et al.Interferon-gamma is atherapeutic target molecule for prevention of postoperative adhesionformation.Nat Med,2008,14(4):437-441.
  • 10Holschneider CH,Nejad F,Montz FJ.Immunomodulation withinterleukin-10 and interleukin-4 compared with ketorolactromethamine for prevention of postoperative adhesions in a murinemodel.Fertil Steril,19997,1(1):67-73.

共引文献24

同被引文献49

引证文献3

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部