经鼻靶向给予降钙素基因相关肽对蛛网膜下腔出血后海马区细胞凋亡的影响

Effect of Intranasal Calcitonin Gene-related Peptide on Apoptosis in Hippocampus of Subarach-noid Hemorrhage

目的探讨经鼻靶向中枢给予降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对海马细胞凋亡和脑损伤的作用。方法枕大池2次注血法制作蛛网膜下腔出血(subarach-noid hemorrhage,SAH)模型。将48只♂Wistar大鼠随机分为正常对照组、SAH组、生理盐水(NS)+SAH组(经鼻给予NS)、CGRP+SAH组(经鼻给予CGRP),每组12只。于第2次注血3 d后,采用免疫荧光技术检测海马组织中Bcl-2和caspase-3的蛋白表达。结果免疫荧光染色显示Bcl-2和caspase-3在正常对照组大鼠海马组织中只观察到极少量表达;SAH组、NS+SAH组Bcl-2和caspase-3蛋白表达明显增多;与SAH组和NS+SAH组相比较,经鼻给予CGRP治疗后,Bcl-2表达显著升高,caspase-3表达水平显著降低,差异均具有统计学意义(P<0.01)。结论 CGRP经鼻靶向中枢给药能有效抑制SAH大鼠海马组织细胞凋亡,对脑损伤具有显著保护作用。

OBJECTIVE To investigate the effect of intranasal calcitonin gene-related peptide（CGRP） on hippocampal apoptosis and cerebral injury. METHODS Subarach-noid hemorrhage（SAH） model was established by cisterna double injection of fresh autologous arterial blood. Forty-eight male Wistar rats were randomly divided into normal control group, SAH group, normal saline（NS）＋SAH group（intranasal NS） and CGRP＋SAH group（intranasal CGRP）（n=12）. The expression of Bcl-2 and caspase-3 in hippocampal tissues was detected by immunofluorescence technique on the 3 rd day after the second injection. RESULTS Immunofluorescence staining results showed that the Bcl-2, caspase-3 expressed very small amounts in normal control group, it was obviously increased in SAH and NS＋SAH group. Compared with SAH and NS＋SAH group, intransal CGRP could significantly rise the expression of Bcl-2（P〈0.01） and decrease the expression of caspase-3 level significantly（P〈0.01）. CONCLUSION Intransal CGRP can inhibit the apoptosis of hippocampal tissue cells effectively and have a significant protective effect on brain injury in SAH rats.