117例甲状腺结节FNAC检查与BRAF基因联合检测的结果分析

Fine-needle aspiration cytology combined BRAF V600E testing of thyroid nodules: analysis of 117 cases

目的探讨超声引导下甲状腺细针穿刺细胞学检查(fine needle aspiration cytology,FNAC)与丝/苏氨酸特异性激酶(serine-threonine protein kinase,BRAF)基因V600E联合检测对于甲状腺结节的临床应用价值。方法收集117例甲状腺结节患者,术前分别行FNAC检查和BRAF V600E突变检测,并将其结果与术后病理诊断结果进行对比分析。结果术前FNAC与BRAF V600E突变联合检测的符合率为92.3%(108/117),灵敏度为88.5%(69/78),均高于单独的BRAF V600E突变检测,差异有统计学意义(P<0.05)。两者联合检测的灵敏度也高于单独的FNAC检查,差异有统计学意义(P<0.05)。结论甲状腺FNAC细胞学评估与BRAF基因联合检测可提高甲状腺结节的术前诊断率,并对临床有重要的指导意义。

Purpose To evaluate the clinical application value of a joint detection that combines ultrasound guided fineneedle aspiration cytology with serine-threonine protein kinase（BRAF V600E） in thyroid nodules. Methods 117 patients with thyroid nodules which underwent FNAC and BRAF V600E test were collected before operation. The results were compared with postoperative pathological diagnosis. Results The consistency of preoperative FNAC and BRAF mutation was 92. 3%（108/117） and sensitivity was 88. 5%（69/78）,both of which were higher than the single BRAF mutation detection,and the difference was statistically significant. The sensitivity of combine detection was also higher than the single FNAC detection,and the difference was statistically significant. Conclusion Preoperative diagnosis rate can be improved by a joint detection that combines FNAC with BRAF V600E mutation detection,and the method has important guiding significance for clinical practice.