番茄SlNAC71基因克隆及表达分析

Cloning and Expression Analysis of SlNAC71 Gene in Tomato

NAC转录因子在植物逆境应答中具有重要的作用。本研究从番茄中克隆了一个NAC基因Sl NAC71。该基因编码区长1 059 bp,编码352个氨基酸,预测分子量约为39.51 k D,等电点为8.40。推测的氨基酸序列中含有1个高度保守的NAM结构域。Sl NAC71基因序列具有mi R164靶序列识别位点。亚细胞定位预测Sl NAC71蛋白定位于细胞核。进化树分析发现,Sl NAC71和At NAC分为一个分支。组织特异性表达模式分析表明在检测的所有组织中Sl NAC71都有表达,在叶中表达量最高,在茎中表达量最低。Sl NAC71基因的表达受高盐诱导,说明番茄Sl NAC71基因可能参与番茄对高盐胁迫的应答。

NAC（NAM, ATAF, and CUC） transcription factors play important roles in plant stress responses. In this study, a NAC gene named as SlNAC71 was cloned from tomato. The full length of SlNAC71 was 1 059 bp.encoding 352 amino acids, with a predicted molecular mass of 39.51 k D and a basic isoelectric point of 8.40.There was a highly conserved NAM domain in the predicted amino acid sequence. SlNAC71 gene sequence had a mi R164 target recognition site. Subcellular localization predicted that SlNAC71 was located in the nucleus.Phylogenetic tree analysis found that SlNAC71 clustered with At NAC. Tissue-specific expression profiles analysis showed that SlNAC71 was nearly expressed in all detected tissues, with the highest expression level in leaves and the lowest in stem. The expression of SlNAC71 was induced by salt stress, indicating that this gene might be involved in the response of tomato to salt stress.