甜菜NAC转录因子BvNAC46基因的克隆及植物表达载体的构建

Cloning and Plant Expression Vector Construction of BvNAC46 Gene in NAC Transcription Factor from Sugar Beet

以干旱处理的强抗旱甜菜品种HI0466为材料,通过RT-PCR技术克隆到一个甜菜NAC类转录因子基因的cDNA序列,命名为BvNAC46(GenBank登录号:XM_010689163.2)。序列分析表明,该基因序列全长1 047 bp,包含一个576 bp的开放阅读框(ORF),编码191个氨基酸,为稳定的疏水性蛋白,推测理论分子量为88.725 kD,亚细胞定位于细胞核中。含有一个NAM保守结构域,具有NAC转录因子的典型特征。将该基因的cDNA编码序列连接到植物表达载体pCAMBIA2301中,成功构建了甜菜BvNAC46基因的植物表达载体pCAMBIA2301-BvNAC,为进一步通过转基因技术研究该基因的功能提供参考。

Taking strong drought-resistant varieties HI0466 of sugar beet under drought treatment as material,the cDNA sequence of NAC transcription factor of sugar beet named Bv NAC46（Gen Bank accession number：XM_010689163.2） was cloned by RT-PCR method. The sequence analysis indicated that the gene was 1 047 bp in whole length, including an open reading frame（ORF） of 576 bp, and encoded 191 amino acids. It was a stable hy drophobic protein, speculated with 88.725 kD theoretical molecular weight which the subcellular localization was in nucleus. The gene contained a NAM conserved domain and showed the typical characteristics of NAC transcription factor. The cDNA coding sequence of that gene was connected into the plant expression vector pCAMBIA2301 and the study successfully constructed the plant expression vector of p CAMBIA2301-BvNAC of the Bv NAC46 gene in sugar beet, which might provide reference for the research on gene function through transgenic technology.