水稻材料IR65482抗稻瘟病基因鉴定与定位

Identification and Mapping of a Blast Disease Resistance Gene in Rice Line IR65482

水稻材料IR65482对不同地区的稻瘟菌小种具有广谱抗性,已知其第6号染色体上具有一个抗稻瘟病病基因Pi40(t)。本研究应用极端分离混合池重测序策略,对IR65482抗稻瘟病基因进行鉴定,并在其第11号染色体上鉴定到另一个抗稻瘟病基因。进一步利用IR65482与日本晴配置的F2群体进行基因定位,将IR65482抗稻瘟病基因定位在水稻第11染色体末端InDel标记OSL3-2和OSL3-5之间约425 kb的区间。本研究结果对利用IR65482开展水稻抗稻瘟病育种具有指导意义,也为后续克隆IR65482的抗病基因提供了理论依据。

Rice line IR65482 has broad-spectrum resistance to Magnaporthe oryzae from different areas, and it has been known that there is a blast resistance gene Pi40（t） on its chromosome 6. In the present study, an extreme separation hybrid pool re-sequencing strategy was used to identify IR65482 resistant blast genes and to identify another rice blast resistance gene on chromosome 11. The F2 population derived from a cross between IR65482 and Nipponbare was further used for gene mapping, and the IR65482 blast resistance gene was mapped in a 425 kb region between In Del markers OSL3-2 and OSL3-5 at the end of chromosome 11. Our results could provide guidance for application of IR65482 in breeding rice varieties with blast resistance and theoretical reference for the cloning of IR65482 blast resistance gene in the future.