铁皮石斛快繁优化及不同时期生物碱积累的研究

Study on the Rapid Propagation Optimization and Alkaloid Accumulation in Different Periods of Dendrobium officinale

本研究以铁皮石斛无菌苗茎段为实验材料优化茎段培养的最适培养基,并对其愈伤组织形成后的10 d、15 d、20 d、25 d、30 d、35 d、40 d等不同时期的生物碱的含量进行测定。结果表明,以MS为基本培养基,原球茎诱导的最佳激素组合为NAA 1.5 mg/L+6-BA 0.5 mg/L,平均诱导率为95%;原球茎增殖的最佳激素组合为6-BA 1 mg/L+NAA 1 mg/L,原球茎分化的最佳激素组合为6-BA 5 mg/L+NAA 1 mg/L,平均分化率可达80%;生根壮苗的最佳组合为:IBA 1.5 mg/L+香蕉泥100 g/L+活性炭0.1%,其生根率为100%;愈伤组织形成后30 d左右时其总生物碱含量最高。

In this study, aseptic seedling stem sections of Dendrobium officinale were used as experimental materials to optimize the optimal medium of stem segment culture, and determine the alkaloid content in different periods after the callus formed, including 10 d, 15 d, 20 d, 25 d, 30 d, 35 d, and 40 d. The results showed that with MS as the basic medium, the most suitable hormone combination for protocorm induction was 6-BA 0.5 mg/L＋NAA 1.5 mg/L, and average induction rate was 95%. The optimal hormone combination for protocorm proliferation was MS＋6-BA 1 mg/L＋NAA 1 mg/L, and for protocorm differentiation, it was MS＋6-BA 5 mg/L＋NAA 1 mg/L,which the differentiation rate reached 80%. The best combination for rooting and proliferation was MS ＋IBA1.5 mg/L＋mashed banana 100 g/L＋active carbon 0.1%, and the rooting rate was 100%. Total alkaloids contents reached the highest in 30 d after the formation of callus.