黄连素对肝癌细胞系HepG2细胞体外迁移影响及其机制研究

Effect and mechanism of berberine on migration of hepatocellular carcinoma cell line HepG2

目的:探讨黄连素对肝癌细胞系HepG2细胞体外迁移的作用及其机制。方法:通过MTT实验,检测不同浓度的黄连素对HepG2细胞存活的影响。利用细胞划痕迁移实验,观察低浓度黄连素(10μmol/L)对HepG2细胞体外迁移的影响以及3-MA对黄连素调控HepG2细胞体外迁移作用的影响。采用Western blot实验,证实低浓度黄连素引起HepG2细胞发生自噬。结果:高浓度黄连素(25μmol/L和50μmol/L)显著抑制HepG2细胞存活,低浓度黄连素未见明显变化。低浓度黄连素抑制HepG2细胞体外迁移并且引起HepG2细胞发生自噬,而3-MA能够逆转黄连素抑制HepG2细胞迁移的作用。结论:低浓度黄连素通过自噬抑制HepG2细胞体外迁移。

Objective：To investigate the effect and mechanism of berberine on migration of hepatocellular carcinoma cell line HepG2.Method：Effcet of berberine in different concentration on HepG2 cell viability was detected by MTT.Cell migration assay was performed to demonstrate the effect of berberine in a low concentration（10 μmol/L）on HepG2 cell migration and role of 3-MA in the effect of berberine on HepG2 cell migration in vitro.10μmol/L berberine inducing HepG2 cell autophagy was demonstrated by western blot.Results：Berberine in high concentrations（25 μmol/L 和 50 μmol/L）significantly inhibits HepG2 cell viability,but 10 μmol/L berberine doesn＇t.10 μmol/L berberine inhibits HepG2 cell migration in vitro and induces HepG2 cell autophagy.3-MA reverses the effect of berberine on HepG2 cell migration.Conclusion：Berberine in a low concentration inhibits HepG2 cell migration in vitro via autophagy.