转SoSUT5基因甘蔗的生长特性

Growth characteristics of SoSUT5 transgenic sugarcane

【目的】研究甘蔗蔗糖转运蛋白So SUT5基因对甘蔗主要农艺性状和相关生理生化特性的影响,为评价转基因甘蔗的生长情况及验证So SUT5基因的功能提供参考。【方法】通过PCR扩增和测序验证6个转So SUT5基因甘蔗品系(T1、T2、T3、T6、T9和T11),以野生型甘蔗B8植株为对照,在苗期、分蘖期、伸长期和成熟期分别测定甘蔗的株高、茎径、叶绿素相对含量和+1叶叶面积,成熟期时测定蔗茎的可溶性糖含量。【结果】通过PCR扩增测序获得6个转So SUT5基因甘蔗品系的目的基因序列,表明导入的So SUT5基因已整合到甘蔗基因组DNA中。除T6外,其他5个转基因甘蔗品系的株高、茎径和+1叶叶面积均高于对照,其中成熟期时5个转基因甘蔗品系的茎径和+1叶叶面积均显著高于对照(P<0.05,下同),T1、T2和T9的株高及T1、T2和T11的叶绿素相对含量显著高于对照。成熟期时6个转So SUT5基因甘蔗品系的可溶性总糖含量均高于对照,其中T2、T9和T11的可溶性总糖含量极显著高于对照(P<0.01),分别比对照高40.3%、40.3%和33.4%。【结论】So SUT5基因在甘蔗中过表达可促进甘蔗生长,并可提高蔗茎的可溶性总糖含量。由此推测,该基因可能与甘蔗节间糖分积累有关,且对甘蔗生长起促进作用。

【Objective】The present study was carried out to investigate the influence of sucrose transporter So SUT5 gene on main agronomic traits and related physiological and biochemical characteristics of sugarcane,and provide references for evalua-ting growth of the transgenic sugarcane and verifying the function of six So SUT5 genes.【Method】The So SUT5 transgenic sugarcane lines（T1,T2,T3,T6,T9 and T11）were verified by PCR amplification and sequencing.Taking wild sugarcane B8 plant as control,agronomic characters such as plant height,stem diameter,relative chlorophyll content and ＋1 leaf area were measured at seedling stage,tillering stage,elongation stage and mature stage respectively,and soluble sugar content in stem were determined at mature stage.【Result】Six target gene sequence of So SUT5 transgenic sugarcane lines were obtained by PCR amplification and sequencing,indicating that the introduced So SUT5 gene has been integrated into sugarcane genomic DNA. Except for line T6,the plant height,stem diameter and ＋1 leaf area in the other five lines were higher than those of control. Among them,the stem diameter and ＋1 leaf area of five transgenic sugarcane lines were significantly higher than those of control at mature stage（P〈0.05,the same below）,the plant height of T1,T2 and T9 and the relative chlorophyll content of T1,T2 and T11 were significantly higher than those of control. The soluble sugar content in the six transgenic lines were higher than the control at mature stage,among which,those of T2,T9 and T11 were extremely higher than that of control（P〈0.01）,and were 40.3%,40.3% and 33.4% higher than that of control respectively.【Conclusion】Overexpression of So SUT5 gene in sugarcane can promote sugarcane growth and increase soluble sugar content. Therefore,it is inferred that this gene is related to sugar accumulation in the sugarcane internodes and may promote the growth of sugarcane.