摘要
CRISPR-Cas系统是一种靶向基因编辑工具,操作简单,逐渐取代人工锌指核酸酶(ZFN)和类转录激活因子效应物核酸酶(TALEN)而成为近年的研究热点。Cas9蛋白能在一段小的RNA引导下特异性结合并切割DNA,在基因组结构和功能学研究中发挥重要作用。后来发现的Cas13等蛋白能特异性结合和编辑RNA,开启了转录组研究的新篇章。我们对CRISPR-Cas系统近年的研究发现做了简要概述,并对该系统作为一种工具在基础研究、生物工程、疾病治疗上的应用进行了总结。
CRISPR-Cas systems dramatically simplified and revolutionized the field of genome engineering. Cas9 can be guided to target specific genomic locations by a short RNA sequence. Genome editing has enabled broad advances and novel approaches in studies of gene function and structure. Recent studies have used other CRISPRCas systems like Cas13 for RNA-based manipulations, leading to novel applications of CRISPR-Cas systems as programmable transcriptional regulators. Here we discussed recent discoveries of CRISPR-Cas systems including some new members in this family and their applications in basic research to biotechnology and therapies.
作者
俞珺瑶
杜华平
YU Jun-Yao;DU Hua-Ping(Department of Hematology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China)
出处
《生物技术通讯》
CAS
2018年第3期422-429,共8页
Letters in Biotechnology
基金
浙江省教育厅一般科研项目(Y201635935)
