黄芪多糖对抑郁大鼠海马NF-κB信号通路的影响

Effects of astragalus polysaccharide on hippocampal NF-κB signaling in rats with depressive behaviors

目的 观察黄芪多糖(astragalus polysaccharide,APS)对抑郁大鼠海马NF-κB信号通路的影响。方法 Wistar大鼠随机分为正常组、抑郁组、APS低、高剂量组(200、400 mg·kg^(-1)·d^(-1))。采用慢性轻度不可预见性应激法(chronic unpredictable mild stress,CUMS)制备抑郁大鼠模型,并分别给予APS低、高剂量组大鼠不同剂量的APS干预4周。通过糖水摄取实验、强迫游泳实验、旷场实验评价大鼠抑郁行为。通过检测海马NF-κB p65、磷酸化NF-κB p65(p-NF-κB p65)、磷酸化IκBα(p-IκBα)、NF-κB p65 DNA结合活性、TNF-α、IL-1β、IL-6水平,评价大鼠海马NF-κB信号通路活性。结果 与正常组比较,抑郁组大鼠糖水摄取实验中糖水摄取量明显下降、强迫游泳实验中静止不动时间明显延长,海马组织中NF-κB信号通路活性明显升高。APS干预明显升高了抑郁大鼠糖水摄取量、缩短了游泳实验的静止不动时间。同时,APS干预明显抑制了抑郁大鼠海马组织NF-κB信号通路的活性。结论 APS可减轻CUMS引起的大鼠抑郁行为,其机制可能与抑制大鼠海马NF-κB信号通路活性有关。

Aim To investigate the effects of astragalus polysaccharide（APS） on depressive behaviors and hippocampal NF-κB signaling in rats with depression.Methods Wistar rats were randomly divided into control group,depressive group,APS-low（200 mg·kg（-1）·d（-1）） group and APS-high（400 mg·kg（-1）·d（-1））group. Depressive behaviors were induced by chronic unpredictable mild stress（CUMS） in rats. After treated with APS,depressive behaviors were valuated by open field test,forced swim test and sucrose preference test. Levels of NF-κB p65,phosphorylated NF-κB p65（p-NF-κB p65）,phosphorylated IκBα（p-IκBα）,NF-κB p65 DNA binding activity,TNF-α,IL-1β and IL-6 were measured to assess the activity of NF-κB signaling pathways. Results Compared to control group,rats in depressive group had less sucrose intake in sucrose preference and longer immobility time in forced swim test,as well as increased hippocampal NF-κB signaling activity. However,APS treatment dose-dependently alleviated depressive-like behaviors and inhibited the activation of NF-κB signaling induced by UCMS. Conclusion The antidepressant effects of APS might be associated with the inhibition of hippocampal NF-κB signaling pathway.