Orexin-B对正常大鼠苍白球神经元自发放电及运动行为的影响

EFFECT OF OREXIN-B ON SPONTANEOUS DISCHARGE OF GLOBUS PALLIDUS NEURONS AND MOTOR BEHAVIOR IN NORMAL RATS

目的探究orexin-B对正常大鼠苍白球神经元自发放电及运动行为的影响。方法分别采用体内细胞外电生理记录和提升躯体摇摆实验(EBST)等方法,观察苍白球给予orexin-B后其神经元自发放电以及大鼠运动行为的变化。结果在苍白球记录的18个基础放电频率为(18.34±2.07)Hz的神经元,微压力给予orexin-B可使其中15个神经元的放电频率增加(22.06±3.65)%(t=5.35,P<0.01),orexin-B对自发放电频率的兴奋效应与生理盐水对照组相比差异有统计学意义(z=4.286,P<0.01)。EBST显示单侧苍白球微量注射orexin-B可使大鼠发生明显的对侧偏转,与生理盐水对照组相比差异有统计学意义(z=2.098,P<0.05)。结论 Orexin-B可明显兴奋正常大鼠苍白球神经元,进而参与大鼠运动行为的调控。

Objective To investigate the effect of orexin-B on the spontaneous discharge of globus pallidus neurons and motor behavior of in normal rats. Methods The methods of in vivo extracellular electrophysiological recordings and elevated body swing test（EBST）were used to measure the changes in spontaneous discharge of globus pallidus neurons and motor behavior in rats after the administration of orexin-B. Results Among the 18 globus pallidus neurons with a recorded resting discharge frequency of（18.34±2.07）Hz,15 showed a significant increase in discharge frequency after micro-pressure administration of orexinB（22.06±3.65）（t=5.35,P〈0.01）,and there was a significant difference in the excitatory effect on discharge frequency between the orexin-B group and the normal saline control group（z=4.286,P〈0.01）.EBST showed that micro-injection of orexin-B in the unilateral globus pallidus induced significant contralateral swing,which was significantly different from that in the normal saline control group（z=2.098,P〈0.05）. Conclusion Orexin-B significantly increases the excitability of globus pallidus neurons in normal rats and thus regulates the motor behavior of normal rats.