MiR-192/-215靶向BIVM基因调控胃癌细胞生物学功能

MiR-192/-215 Regulates the Biological Functions of Gastric Cancer Cells by Targeting BIVM

【目的】研究miR-192/-215通过靶基因BIVM调控人胃癌发生发展的机制。【方法】首先利用靶基因预测网站、基因芯片、实时荧光定量PCR技术筛选出miR-192/-215的靶基因BIVM,然后构建双荧光素酶报告质粒,验证BIVM为miR-192/-215靶基因。随后通过转染BIVM-siRNA干扰BIVM表达后,检测其对胃癌细胞增殖、凋亡的作用。8只裸鼠随机分为BIVM-siRNA实验组和NSC-siRNA对照组,将转染BIVM-siRNA的胃癌细胞种植于裸鼠皮下,观察BIVM对胃癌细胞体内成瘤能力的影响。【结果】基因芯片及荧光定量PCR筛选出BIVM为miR-192/-215的靶基因,双荧光素酶报告系统确认miR-192/-215靶定BIVM。细胞增殖实验发现BIVM可促进胃癌细胞增殖(P<0.05),流式细胞凋亡实验显示BIVM能抑制胃癌细胞凋亡(P<0.05),小鼠成瘤实验证实BIVM可促进胃癌细胞体内生长(P<0.05)。【结论】BIVM在胃癌中发挥致癌基因的作用,miR-192/-215调控BIVM表达从而促进胃癌的发展进程。

【Objective】To investigate the molecular mechanism of miR-192/-215 targeting BIVM in human gastric cancer.【Methods】First,BIVM was the target gene of miR-192/-215 screened by the target gene prediction in solico and gene microarrays. Real-time quantitative PCR verified the results of the microarrays. Then the double-luciferase reporter plasmids were constructed to test that BIVM was the target gene of miR-192/-215. Subsequently,BIVM-siRNA was transfected,to know the effects of BIVM-siRNA on proliferation and apoptosis of gastric cancer cells. 8 nude mice were randomly divided into two groups：BIVM-siRNA experimental group and NSC-siRNA control group. Gastric cancer cells transfected with BIVM-Si RNA were implanted under the skin of nude mice to observe the effect of BIVM on the tumorigenicity of gastric cancer cells.【Results】BIVM was screened as miR-192/-215 target gene by gene microarrays and quantitative PCR. Double-luciferase reporting assays were performed to identify the BIVM as miR-192/-215 target gene. The cell proliferation assays showed that BIVM promoted the proliferation of gastric cancer cells（P0.05）. Test of flow cytome-try showed that BIVM inhibited the apoptosis of gastric cancer cells（P0.05）. Mouse tumorigenesis test confirmed that BIVM could promote gastric cancer cells growth in vivo（P0.05）.【Conclusion】BIVM plays a role in the carcinogenesis of gastric cancer,and miR-192/-215 targeting BIVM promotes the development of gastric cancer.