不同品种红树莓果实发育过程中抗氧化性的研究

Study on the antioxidant activity of different varieties of red raspberry fruit during the development process

为阐明红树莓果实发育过程中的抗氧化规律,明确抗氧化性强的时期和品种,以4个品种4个时期的红树莓果实为材料,对抗氧化酶活性、总酚含量及其对DPPH自由基的清除作用进行研究。结果表明:4个红树莓品种果实从青果到深红果发育过程中SOD活性随发育程度增加而升高,‘海尔特兹’深红果最高为36.99U/(g·min)FW;CAT活性随发育程度增加而先增高后降低,‘海尔特兹’-黄果最高为41.12U/(g·min)FW;POD活性随发育程度增加而降低,‘海尔特兹’-青果最高为9.83U/(g·min)FW。4个红树莓品种果实发育过程中总酚含量在7.34~41.88mg/g之间;对DPPH自由基的清除率在7.22%~33.83%之间,随发育程度增加而先降低后升高。结论:‘海尔特兹’的抗氧化酶活性最高,SOD、CAT、POD活性最高的时期为深红果、黄果、青果;‘秋福’与‘海尔特兹’的总酚含量高,青果时高;‘秋福’与‘菲尔杜德’的清除率最高,青果与深红果时高。

In order to elucidate the antioxidant regularity during the development process of red raspberry fruit, identify the period and variety of antioxidants are strong.The four varieties of red raspberry fruits at four developmental stages were used as materials, antioxidant enzymes activity, total phenolic content and its scavenging effect on DPPH free radical were studied. The SOD activity of four varieties of red raspberry fruit increased with the development stage of fruit, the highest SOD activity of Heritage-deep red fruit was 36.99 U/（g·min） FW; the activity of CAT with the degree of development of the fruit increased first and then decreased, the highest CAT activity of Heritage-yellow fruit was 41.12 U/（g·min） FW;the activity of POD with the degree of development of the fruit gradually decreased, and the highest POD activity of Heritage-green fruit was 9.83 U/（g·min） FW. The total phenolic content of four varieties of red raspberry fruits at different developmental stages was between 7.34—41.88 mg/g FW and DPPH clearance rate between 7.22% to 33.83%,all with the development of the fruit increased first and then increased.Conclusion： The activity of antioxidant enzymes was the highest in SOD, CAT, POD activity of the highest period for the deep red fruit, yellow fruit, green fruit;‘Au-tumn Bliss’ and ‘Heritage’ the highest total phenol content, higher when the green fruit;‘Au-tumn Bliss’ and ‘Fertod Zamatos’ the highest rate of clearance, green fruit and deep red fruit when higher.