摘要
氨基酸分析在生物化学、蛋白质组学、临床医学、食品科学和化工等领域有着广泛的应用,也是分析化学中的一个研究热点。对无需衍生化的直接检测氨基酸和需要衍生化的间接测定氨基酸的2种方法进行综述。对于直接检测法,主要从检测器方面论述近年来主要的检测非衍生化氨基酸的一些基本理论及实例;对于间接检测法,从衍生试剂及相应衍生物的检测器方面分析各种衍生试剂的优劣及衍生化方法,并对氨基酸的分析进行了展望。
Amino acid analysis has been widely applied in biochemistry,proteomics,clinical medicine,food science,chemical industry and other fields,it is also a hotspot in analytical chemistry. This paper reviews two methods of analyzing amino acids including direct analysis and indirect analysis. Direct analysis method mainly discusses basic theories and examples of the detection of non-derivative amino acids with corresponding detector in recent years. Indirect analysis method compares various derivatization reagents and derivative method. Meanwhile,the development trends of the analysis method of amino acids are also discussed.
作者
丁松
黄和
胡燚
DING Song;HUANG He;HU Yi(School of Pharmaceutical Sciences,Nanjing Tech University, Nanjing 211800, China)
出处
《生物加工过程》
CAS
2018年第3期12-21,共10页
Chinese Journal of Bioprocess Engineering
基金
国家自然科学基金(21676143)
江苏省青蓝工程
江苏高校优秀科技创新团队计划
关键词
氨基酸
衍生
非衍生
检测器
amino acids
derivatization
non-derivative
detector
