栀子豉汤提取物对谷氨酸诱导PC 12细胞损伤的保护作用

The Protective Effect of Zhizichi Decoction on the Apoptosis of PC 12 Cells Induced by Glutamic Acid

目的研究栀子豉汤提取物对谷氨酸诱导PC 12细胞损伤的保护作用。方法通过谷氨酸诱导PC 12细胞建立细胞损伤模型,采用四甲基偶氮唑蓝(MTT)比色法检测细胞存活率;化学检测法测定乳酸脱氢酶(LDH)释放量;Annexin V-FITC/propidium iodide(PI)双染法流式细胞术(flow cytometry,FCM)检测细胞凋亡率;流式细胞仪检测PC 12细胞内活性氧(ROS)水平,酶标仪检测超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的含量,初步探讨栀子豉汤提取物的抗氧化机制。结果栀子豉汤可显著降低由谷氨酸诱导的细胞损伤,提高细胞存活率,减少LDH释放量,增加SOD、GSH活性,抑制ROS的生成。结论栀子豉汤提取物具有减轻由谷氨酸诱导的PC 12细胞损伤的作用,其机制可能与抗氧化应激损伤有关。

OBJECTIVE To explore the protective effect of Zhizichi Decoction extract against glutamic acid-induced neurotoxicity in PC 12 cells. METHODS PC 12 cells were treated with glntamic acid to establish the cell model. Cell viability and membrane per- meability were examined by MTT assay and LDH kit. The apoptosis rates were measured by flow cytometry using fluorescein labeling FITC-Annexin V/PI. The production of ROS was determined by flow cytometry. The levels of GSH and SOD in the cells were deter- mined by kit respectively. RESULTS Zhiziehi Decoction extract significantly decreased the contents of LDH, enhanced the activities of SOD and GSH induced by glutamate. In addition, Zhizichi Decoction extract treatment decreased the intracellular ROS generation. CONCLUSION Zhizichi Decoction extract can significantly ameliorate glutamic acid induced PC 12 cells injury. The mechanism may be partly through anti-oxidative stress.