Crk在胃癌中的表达及其临床意义

Clinical significance of Crk expression in gastric cancer

目的:探讨接头蛋白Crk在胃癌组织中的表达及其与胃癌临床病理因素及预后的关系。方法 :采用免疫组织化学方法检测368例胃癌病人肿瘤组织和配对癌旁组织Crk蛋白表达水平,分析其表达与临床病理因素及预后之间的关系。结果:与癌旁组织相比,胃癌组织中Crk蛋白表达水平明显升高(P<0.05)。其表达水平与肿瘤大小(P<0.05)、浸润深度(P<0.05)、淋巴结转移(P<0.05)、TNM分期(P<0.05)密切相关。Crk蛋白表达水平越高,病人预后越差(P<0.05)。多因素分析表明,Crk蛋白是影响胃癌病人预后的独立危险因素。结论:Crk蛋白在胃癌的发生、发展中起重要作用,可作为预测胃癌预后的分子标志物。

Objective To evaluate the expression of adaptor protein Crk in gastric cancer and the associations with clinicopathological characteristics and prognosis of gastric cancer.Methods The expression of Crk protein of matched tumor and adjacent tissues in 368 patients with gastric cancer was analyzed by immunohistochemistry.The relation of Crk protein expression with clinicopathological characteristics and clinical outcome was evaluated.Results Compared with adjacent non-tumor tissues,Crk protein was significantly up-regulated in tumor tissues（P〈0.05）.An elevated Crk protein expression statistically related with aggressive clinicopathological characteristics significantly including larger tumor size（P〈0.05）,deeper local invasion（P〈0.05）,more lymph node metastasis（P〈0.05） and advanced TNM stage（P〈0.05）.Gastric cancer patients with higher expression of Crk protein had poorer prognosis（P 〈0.05）.Multivariate analysis showed that higher level of Crk protein was an independent unfavorable prognostic factor of gastric cancer.Conclusions Crk protein may be associated with tumor progression in gastric cancer and may predict unfavorable prognosis of gastric cancer as a novel molecular marker.