阿伦磷酸钠缓释微球局部抗骨质疏松效果研究

Local application of Alendronate sodium sustained-release microspheres in osteoporosis

目的探讨阿伦磷酸钠缓释微球对骨质疏松大鼠局部的缓释效果。方法将50只骨质疏松大鼠随机分为4组,A组为对照组,不予任何干预;B组为全身给药组,通过灌胃方式给药;C组为空白微球组,D组为载药微球组,C、D组均通过注射方式予左股骨中部注入微球。8周后处死各组大鼠,取左股骨,D组同时取右侧股骨,测量各组大鼠左股骨骨密度,并测量D组大鼠右股骨骨密度。同时对各组大鼠左侧股骨骨应力进行测量比较,并对D组大鼠右侧股骨进行骨应力测量。结果 B组、D组较A组骨密度增加(P<0.05),C组与A组骨密度差异无统计学意义(P>0.05),D组骨密度高于B组(P<0.05)。D组左侧股骨骨密度高于右侧(P<0.05)。股骨应力学检测结果提示,B组、D组较A组三点弯曲最大载荷值增加(P<0.05),C组与A组三点弯曲最大载荷值差异无统计学意义(P>0.05),D组三点弯曲最大载荷值高于B组(P<0.05)。D组左侧股骨三点弯曲最大载荷值高于右侧(P<0.05)。结论应用阿伦磷酸钠缓释微球可增强骨质疏松大鼠局部的骨密度。

Objective To investigate local application of Alendronate sodium sustained release microspheres（ASSRM） on osteoporosis and its therapeutic effect. Methods A total of 40 osteoporotic rats were randomly divided into 4 groups： group A in which rats did not receive any intervention, group B in which drugs were systemically administrated, group C in which rats received injection of blank microsphere at left femur, and group D in which drug loaded microsphere group were injected into the left of femur. Femurs were harvested 8 weeks after treatments. Bone density was measured. Bone stress was manifested by maximum load of three-point bending. Results No obvious difference in bone density and stress was observed between group C and A（P〉0.05）. Rats in group B and group D experienced increased bone density and stress compared with group A（P〈0.05）, while femurs in group D had improved bone density and stress when compared with that in group B（P〈0.05）. Inside group D, ASSRM treated femurs exerted significant upregulation of bone density and stress when compared with contralateral right femur（P〈0.05）. Conclusion Application of Alendronate sodium sustained release microspheres significantly enhances the local bone mineral density and bone stress in osteoporosis.