单抗KMP1的体内抑制膀胱癌EJ细胞株成瘤的动物实验

The Research of Monoclonal Antibody KMP1 Inhibited Bladder Cancer EJ Cell Lines Growth and Metastasis in Vivo

目的研究膀胱癌单抗KMP1的体内抑制EJ细胞成瘤功能.方法免疫组化鉴定单抗KMP1与EJ、EJ-GFP的结合性,荧光活体成像观察KMP1的抑制EJ-GFP裸鼠移植瘤的生长状况,免疫组化和HE染色鉴定移植瘤的肿瘤特征.结果 EJ和EJ-GFP细胞成瘤功能的生长曲线近似,EJ-GFP呈现良好的绿色荧光,EJ和EJ-GFP细胞都能与KMP1结合,且KMP1治疗组移植瘤重量明显低于m Ig G对照组(P<0.001).结论 KMP1能良好的抑制EJ-GFP细胞在裸鼠体内移植瘤生长,KMP1能抑制膀胱癌EJ细胞株体内移植瘤,是一种可能的靶向治疗药物.

Objective To research the monoclonal antibody KMP1 inhibited bladder cancer EJ cell lines growth and metastasis in vivo by bioluminescence imaging. Me thods Immunohistochemistry was used to determine the KMP1 binding to EJ and EJ-GFP cell lines. The xenograft tumor cell growth and distribution were measured by vernier calipers and dynamic in vivo fluorescence imaging. Immunohistochemistry and HE counterstaining researched the feature of the xenograft tumor. Re s ults Cell growth curves of EJ and EJ-GFP cells were similar.EJ-GFP had a green fluorescence. In EJ-GFP nude mouse tumor model, the addition of KMP1 significantly inhibited tumor growth and extended the average life span of nude mice. Both EJ and EJ-GFP cells can bind to KMP1,and the weight of transplanted tumors in the KMP1 treatment group was significantly lower than that of the m Ig G control group（P 〈0.001）.Conclus ion KMP1 has a promising antitumor effect in vivo. It might be valuable for development as a promising targeted agent for bladder cancer.