摘要
目的研究三七皂苷元衍生物,并对其进行抗肿瘤活性筛选.方法人参皂苷Rg1用Smith降解法水解,水解得到的产物用硅胶柱层析分离和纯化.产物结构式利用NMR的数据分析鉴定.化合物(1)和1’-羟基双氧乙基原人参三醇的抗肿瘤活性用CDC25B活性分析和荧光技术检测.结果得到化合物1’-羟基双氧乙基原人参三醇、20(S)-原人参三醇和24,25-烯-3β,6α-二羟基-12,20-(1’2’-双氧异丙叉)双氧丙基-达玛烷.结论化合物(1)是新颖的三七皂苷元衍生物,结构式命名为1’2’-双氧异丙叉双氧丙基原人参三醇,对CDC25B有抑制作用,表明有一定抗肿瘤活性.
Objective To research the derivatives of panax notoginseng sapogenins and their anti-tumor activities. Me thods The ginsenosides Rg1 was treated with Smith degradation. The products were separated and purified by silica gel column chromatography. The structures of the products were determined by NMR spectra. The activity of anti-tumor cells of compound（1） and 1＇-hydroxyethanedioxy PT was detected with CDC25 B activity assay and fluorescence technique. Re s ults 1＇-hydroxyethanedioxy PT, 20（s）-protopanaxatriol（PT）, and24, 25-en-3β, 6 α-dihydroxy-12, 20-（1＇, 2＇-isopropylidenedioxy） propanedioxy-dammarane（1）were isolated and identified. Conclus ion Compound（1）, named 1＇,2＇-isopropylidenedioxy-propanedioxy-propanedioxy, is a novel derivative of panax notoginseng sapogenin with better inhibitory activity against CDC25 B.
作者
李瑞
段文越
邹澄
赵庆
黄丽
周金娜
胡建林
杨为民
LI Rui;DUAN Wen-yue;ZOU Cheng;ZHAO Qing;HUANG Li;ZHOU Jin-na;HU Jian-lin;YANG Wei-min(School of Pharmaceutical Sciences ＆ Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Yunnan Kunming 650500;Yunnan University of Chinese Medicine,Yunnan Kunming 650500,China)
出处
《昆明医科大学学报》
CAS
2018年第5期11-15,共5页
Journal of Kunming Medical University
基金
云南省科技厅-昆明医科大学应用基础研究联合专项重点基金资助项目[2017FE468(-001)]
昆明医科大学研究生创新基金资助项目(2017S070)
