红树林沉积物中芘降解菌群及其代谢质粒转化菌的研究

Effects of the pyrene degradation by microbial consortium and the transformed bacteria in Mangrove sediment

经过富集驯化培养,从红树林表层沉积物中筛选出以多环芳烃芘为唯一碳源和能源的降解菌群YL。该菌群21 d内对50 mg/L芘的降解率可达92.09%。同时采用改良方法提取芘降解菌群YL的质粒,转化至大肠杆菌并通过驯化的方法得到一组以芘为唯一碳源和能源的质粒转化菌群,21 d的芘降解率为85.69%。而受体菌大肠杆菌的芘降解率只有2.01%。由此可以认定该质粒转化菌群具有不俗的芘降解能力,其质粒上携带降解芘的基因。

A microbial consortium named YL which can utilize pyrene as sole carbon and energy source was separated from the sediment of mangrove. YL showed high degradation capability with 92. 09% of 50 mg/L pyrene within 21 days. The plasmid was extracted from the pyrene degrading bacteria consortium YL by the improved method and transformed into Escherichia coli. Through the method of enrichment and culture,the transformed bacteria could utilize pyrene as sole carbon and energy sources. The biodegrading rate of pyrene was 85. 69% within 21 days. While the E. coli only degrade 2. 01% of pyrene within 21 days. Therefore,the transformed bacteria have a good pyrene degradation capability,and the plasmids carry the gene encoded for pyrene biodegradation.