一株抗镉真菌的鉴定及其抗性的初步研究

Identification and preliminary research on the resistance of a Cd-resisting fungus

采用梯度浓度驯化的方法,从河南理工大学煤场土样中分离到一株抗镉真菌,编号为M2。通过传统形态学方法及真菌18S rDNA序列的系统发育分析鉴定,菌株M2为旋孢腔菌属(Cochliobolus)月状旋孢腔菌(Cochliobolus lunatus,有性态),即弯孢霉属(Curvularia Boedin)新月弯孢霉(Curvularia lunata,无性态),其可在镉质量浓度为4000 mg/L的平板上生长。同时还初步探索了菌株M2对重金属铅、铬、铜、锌、钴和镍的耐受性,菌株对7种重金属的抗性强弱顺序为Cd^(2+)(4000 mg/L)>Pb^(2+)(1500 mg/L)>Zn^(2+)(600 mg/L)>Cu^(2+)(400 mg/L)>Ni^(2+)(100 mg/L)>Co^(2+)(80 mg/L)>Cr^(2+)(60 mg/L)。

A strain of Cd-resisting fungus,named M2,was isolated from soil samples near the coal yield by acclimation based on the concentration gradient approach. M2 was identified as Cochliobolus lunatus（ telemorph）,namely Curvularia lunata（ anamorph）,by traditional morphological method and phyogenetic analysis based on 18 S rDNA sequence. This strain was able to grow well on plate containing 4000 mg/L Cd2＋. We also studied the resistance of M2 to heavy metal Pb,Cr,Cu,Zn,Co and Ni. The resistance decreased in the order of Cd2＋（ 4000 mg/L） 〉 Pb2＋（ 1500 mg/L） 〉 Zn2＋（ 600 mg/L） 〉 Cu2＋（ 400 mg/L） 〉 Ni2＋（ 100 mg/L） 〉 Co2＋（ 80 mg/L） 〉 Cr2＋（ 60 mg/L）.