银杏叶提取物对慢性阻塞性肺疾病大鼠T细胞亚群的影响

Effects of Ginkgo biloba Extract on T Cell Subsets in Rats with Chronic Obstructive Pulmonary Disease

目的探讨银杏叶提取物(Ginkgo biloba Extract,GBE)对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠血清、肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中T淋巴细胞亚群的影响。方法将90只雄性Wistar大鼠按照随机数字表法分为正常对照组(A组)、COPD对照组(B组)、GBE早期干预组(C组)、GBE后期干预组(D组)、红霉素早期干预组(E组)及红霉素后期干预组(F组),每组15只。对B、C、D、E和F组采用烟雾吸入、冷空气刺激等复合因素对大鼠进行28 d COPD造模,C和D组分别在1~14 d和29~42 d用GBE干预,E和F组分别在1~14 d和29~42 d用红霉素干预,检测各组血清、BALF前后CD4^+、CD8^+含量,并计算CD4^+/CD8^+比值。结果 B组血清与BALF中CD4^+、CD4^+/CD8^+比值明显低于A组(P<0.05),CD8^+的含量明显高于A组(P<0.05)。C、D两组血清与BALF中CD4^+、CD4^+/CD8^+比值明显高于B组(P<0.05),CD8^+含量明显低于B组(P<0.05);C、D、E和F组之间CD4^+、CD8^+、CD4^+/CD8^+比值进行两两比较,差异无统计学意义(P>0.05),C组BALF中CD4^+的含量明显高于E、F组(P<0.05)。结论 GBE可通过增加COPD大鼠血清及BALF中CD4^+含量,提高CD4^+/CD8^+比值,降低CD8^+含量,实现对免疫功能的调节,从而增强机体免疫力。

Objective To observe the effect of Ginkgo biloba Extract（ GBE） on T lymphocyte subsets in serum and bronchoalveolar lavage fluid（ BALF） of chronic obstructive pulmonary disease（ COPD） rats. Methods Totally 90 male Wistar rats were randomly divided into the normal control group（ Group A）,the COPD control group（ Group B）,GBE early intervention group（ Group C）,GBE late intervention group（ Group D）,erythromycin early intervention group（ Group E）,and erythromycin late intervention group（ group F）,according to random number table method,15 in each group. COPD rat modeling was induced by smog inhalation,cold air stimulation,etc. complex factors in rats of Group B,C,D,E,and F for 28 days. GBE was intervened to rats in Group C and D respectively at day 1-14 and day 29-42. Erythromycin was intervened to rats in Group E and F respectively at day 1-14 and day 29-42. Contents of CD4^＋and CD8^＋in serum and BALF were detected before and after intervention,and CD4^＋/CD8^＋ratio was calculated. Results The contents of CD4^＋and ratio of CD4^＋/CD8^＋in serum and BALF were significantly lower in Group B than in Group A（P〈0. 05）,and the content of CD8^＋was significantly higher in Group B than in Group A（P〈0. 05）. The contents of CD4^＋and ratio of CD4^＋/CD8^＋in serum and BALF in GroupC and D were significantly higher than those in Group B（P〈0. 05）,and the content of CD8^＋was significantly lower than that in Group B（P〈0. 05）. The contents of CD4^＋,CD8^＋,and ratio of CD4^＋/CD8^＋showed no statistical significance among Group C,D,E,and F（P〉0. 05）. And the content of CD4^＋in BALF was significantly higher in Group C than in Group E and F（P〈0. 05）. Conclusion GBE could increase the content of CD4^＋in serum and BALF of COPD rats,elevate the ratio of CD4^＋/CD8^＋,reduce the content of CD8^＋,thus regulating immune function and enhancing the immunity of the body.