离体灭活多孔骨瓣结合BAM骨诱导人工骨修复大鼠颅骨缺损的效果评价

Efficacy of inactivated autologous porous bone flap and BAM bone-induced artificial bone for repairing skull defect in rats

目的研究离体灭活多孔骨瓣结合BAM骨诱导人工骨通过骨诱导成骨修复颅骨骨缺损的作用。方法 72只Wistar大鼠颅骨骨缺损建模后随机分为4组:骨缺损未修复组(CON组)、灭活自体骨瓣支架治疗组(AB组)、BAM骨诱导人工骨材料治疗组(BAM组)和灭活自体骨瓣支架结合BAM骨诱导人工骨材料治疗组(BAM+AB组)。颅骨骨缺损建模术后1、2、3个月取标本,Micro-CT观察颅骨缺损的愈合情况,组织学观察新生骨形成情况。结果离体灭活多孔骨瓣结合BAM骨诱导人工骨可诱导大鼠颅骨缺损的血管与纤维组织再生;离体灭活多孔骨瓣结合BAM骨诱导人工骨可诱导大鼠颅骨缺损成骨发生;BAM骨诱导人工骨在离体灭活多孔骨瓣支撑下可使颅骨缺损更好恢复颅骨外观。结论应用离体灭活自体骨瓣和BAM骨诱导人工骨复合材料修补颅骨缺损,不仅可以促进颅骨愈合,而且避免了材料脆性过大不能成型的弊端,使颅骨外观恢复原貌,达到颅骨表面局部解剖重建要求,是颅骨缺损有效可行的方法 。

Objective To study the effect of BAM bone grafting combined with inactivated autologous porous bone flap in repairing skull defect in rats. Methods Seventy-two Wistar rats with skull defect were randomly divided into control group,inactivated autologous bone flap group（AB group）, BAM bone-induced artificial bone material group（BAM group）, and inactivated autologous bone flap with BAM bone-induced artificial bone group（BAM ＋ AB group）. The bone healing was evaluated with micro-CT and the new bone formation was assessed with histological staining at 1, 2, and 3 months after modeling. Results Inactivated porous bone flap combined with BAM bone-induced artificial bone effectively induced vascular and fibrous tissue regeneration and osteogenesis in the cranial defects. With the inactivated porous bone flap as the scaffold,BAM bone-induced artificial bone obviously promoted the restoration of the skull appearance in the rats with cranial defects.Conclusion Inactivated autologous bone flap group and BAM bone-induced artificial bone material can promote skull healing and restoration of the original skull appearance, and can be used for reconstruction of the local anatomy of the skull surface.