白细胞介素-8基因多态性与小儿支气管哮喘发病率及临床预后的关系

Relationship of interleukin-8 gene polymorphism with asthma incidence and clinical prognosis in children

目的探讨白细胞介素-8基因多态性与小儿支气管哮喘发病率及临床预后的关系。方法选取2015年月6-2017年6月本院确诊支气管哮喘的患者80例作为观察组,并以同期120例健康体检者作为对照组,检测患者外周血IL-8-251SNP基因多态性及炎症因子水平和肺功能。结果观察组IL-8-251SNP基因型为A/A的患者支气管哮喘的风险是T/T的2.6倍,A/T患者支气管哮喘的风险为T/T的1.2倍,含A基因患者支气管哮喘的风险为不含A基因的1.8倍,差异有统计学意义(P<0.05)。基因型为A/A的患者IL-8、TNF-α、IL-6、IL-10水平高于A/T、T/T和Any A基因型的患者,基因型为A/A的患者FEV1/FVC、MEF25、MEF50、MEF25-75低于其他各基因型,差异有统计学意义(P<0.05)。结论 IL-8基因-T-251 A/A增加患者支气管哮喘的易感性,与患者病情严重程度密切相关。

Objective To investigate the relationship of interleukin-8 gene polymorphism with the incidence and clinical prognosis of children with bronchial asthma. Methods 80 patients with bronchial asthma in our hospital from june 2016 to june2017 were selected as the observation group,and 120 healthy subjects were selected as control group in the same period. The IL-8 251 SNP gene polymorphism,the level of inflammatory factors and lung function were detected. Results The risk of bronchial asthma in patients with IL-8-251 SNP genotype as A/A in the observation group was 2. 6 times of that in patients with T/T,that in A/T patients was 1. 2 times of that in T/T patients,and that in patients with A gene was 1. 8 times of that with A gene-free,and the differences were statistically significant（P〈0. 05）. Patients with genotype A/A had higher levels of IL-8,TNF-α,IL-6,IL-10 than patients with A/T,T/T,and Any A genotypes. Patients with genotype A/A had lower FEV1/FVC,MEF25,MEF50,and MEF25-75 levels than that with other genotypes,with the differences statistically significant（P〈0. 05）. Conclusion IL-8 gene-T-251 A/A increases susceptibility to bronchial asthma,and is closely related to the severity of the disease.