p53诱发转录因子FoxM1在卵巢癌中的表达及临床意义

Expression of p53-induced FoxM1 transcription factor in ovarian cancer and the clinical significance

目的研究p53诱发转录因子FoxM1在卵巢癌中的表达及其临床意义。方法首先在卵巢癌患者组织中初步鉴定p53和FoxM1表达;其次在A2780细胞培养过程中添加Nutlin-3、CHX、PFTα调控p53的表达,再鉴定FoxM1表达情况,分析两者相关性;最后,调控p53表达量后研究DPP促使A2790凋亡的能力。结果在卵巢癌患者组织样品中,随着Ⅰ~Ⅳ期分级,p53和FoxM1表达量显著上升;添加Nutlin-3、CHX、PFTα后A2780细胞生长状况无明显的变化,其中CHX和PFTα组p53表达显著抑制,FoxM1也随之明显下降,Nutlin-3组p53表达显著上升,FoxM1也随之上升;在A2780细胞中添加DPP后,p53和FoxM1表达量均显著降低;上调p53表达后,A2780对DPP的敏感性显著增强,诱导细胞凋亡效果明显。结论在卵巢癌细胞中,p53诱发FoxM1的表达,此外p53和FoxM1参与DPP诱导卵巢癌细胞的凋亡途径。

Objective To research the expression of p53-induced FoxM1 transcription factor in ovarian cancer and the clinical significance. Methods The expressions of p53 and FoxM1 were detected in ovarian cancer tissue. 1o53 regulated by Nutlin-3, CHX, and PFTα was added in the course of A2780 cell culture, the expression of FoxM1 was detected, then the correlation was analyzed. The ability of DPP inducing A2790 apoptosis was researched after regulation of IO53 expression. Results In ovarian cancer tissue, the expression levels of p53 and FoxM1 increased significantly with the grade I -IV. After adding Nutlin-3, CHX, and PFTa, the growth status of A2780 cells didn＇t change significantly, p53 expression was inhibited significantly in CHX and PFTα group, FoxM1 decreased significantly. p53 expression in Nutlin-3 group increased significantly, oxM1 also increased. After adding DPP in A2780 cells, the expression levels of p53 and FoxM1 decreased significantly. After upregulating p53 expression, the sensitivity of A2780 to DPP increased significantly, the inducing effect on apop- tosis was obvious. Conclusion In ovarian cancer cells, p53 can induce FoxM1 expression, p53 and FoxM1 are involved in DPP-induced apoptosis of ovarian cancer cells.