摘要
为筛选出对桃蚜Myzus persicae具有高致病性的生防真菌,以从陕西省秦岭原始森林采集到的鳞翅目僵虫虫体中分离获得的5株真菌为研究对象,测定其对桃蚜的致病性并筛选出高致病性菌株表现最佳杀蚜活性时的孢子悬浮液浓度,同时结合形态学及18S rDNA和ITS-rDNA序列分析对致病性最高的病原菌进行鉴定。结果显示,从5株菌株中初步筛选出1株高致病性菌株BQ-63,处理7 d后桃蚜的死亡率为80.33%,校正死亡率为81.58%,僵虫率也达到最高,为80.78%;当菌株BQ-63的孢子浓度为108个/mL时,对桃蚜的致病性达到最高,处理7 d后死亡率为89.53%,校正死亡率为90.10%,僵虫率为89.84%;通过形态学和分子生物学鉴定,确定菌株BQ-63为球孢白僵菌Beauveria bassiana。表明菌株BQ-63对桃蚜具有高致病性,可作为生防真菌进行进一步的研究。
In order to select highly pathogenic biocontrol strains for Myzus persicae(Sulzer), five fungal isolates were isolated from Lepidoptera muscardine cadavers in the primeval forests of Qinling in Shaanxi Province. Based on pathogenicity test, the most highly pathogenic biocontrol strain and its optimal concentration of spore were screened out. Morphological characteristics and molecular identification of the partial sequences of 18 S rDNA and ITS-rDNA were used. The results showed that the best strain, BQ-63, was obtained from the five isolates. After seven-day-treatment, the mortality rate of M.persicae was 80.33%; the corrected mortality was 81.58% and the rate of corpse was 80.78%. Under different spore concentrations of BQ-63, the pathogenicity against M. persicae was measured, and the results showed that the best spore concentration was 108/mL, with a mortality rate, corrected mortality and the rate of corpse of 89.53%, 90.10% and 89.84%, respectively. The strain BQ-63 was identified as Beauveria bassiana by using morphological and molecular methods. The results indicated that strain BQ-63 was highly virulent to M. persicae and could be used as a biocontrol fungus for further research.
作者
田佳
汝冰璐
王颖
成巨龙
李晓宇
安德荣
Tian Jia;Ru Binglu;Wang Ying;Cheng Julong;Li Xiaoyu;An Derong(College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China;Tobacco Company of Shaanxi Province, Xi'an 710068, Shaanxi Province, China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2018年第3期606-613,共8页
Journal of Plant Protection
基金
国家公益性行业(农业)科研专项(K312021301)
陕西省省科委科研专项(K332021006)
农业部农业行业专项(nyhyzx07-051)
关键词
球孢白僵菌
桃蚜
形态学
分子生物学
鉴定
Beauveria bassiana
Myzus persicae
morphology
molecular biology
identification