结肠癌微环境对HMSC-bm形态、增殖、周期及CD34、CD90的影响

Effect of Colon Cancer Microenvironment on the Morphology, Proliferation, Cycle, CD34 and CD90 of HMSC-bm

该文旨在探究不同条件结肠癌微环境对人骨髓间充质干细胞(human mesenchymal stem cells-bone marrow,HMSC-bm)的影响。实验将不同配比的HMSC-bm和SW480细胞经Transwell非接触共培养3天、5天和7天后,通过检测HMSC-bm的显微形态、增殖能力、细胞周期以及细胞表面标志分子CD34、CD90的变化,研究结肠癌微环境对HMSC-bm的影响,同时设立HMSC-bm单独培养组和SW480单独培养组作为对照组。研究发现,随着结肠癌细胞比例增加,共培养时间延长,HMSC-bm细胞的显微形态发生显著改变,HMSC-bm细胞增殖速度加快,且CD34阳性表达率逐渐增多;CD90阳性表达率逐渐减少。这说明,结肠癌微环境可能诱导HMSC-bm细胞发生恶性转化,且该恶性转化与细胞比例和诱导时间有关。

The purpose of this study was to investigate the effects of different conditions of colon cancer microenvironment on human mesenchymal stem cells-bone marrow（HMSC-bm）. In the experiments, the different ratios of HMSC-bm and the colon cancer cell SW480 were non-contact co-cultured with Transwell for 3 d, 5 d and 7 d. The microscopic morphology, proliferative capacity, cell cycle and cell surface marker molecules CD34 and CD90 of each group were tested to investigate the effects of different colon cancer microenvironment on HMSCbm. At the same time, HMSC-bm alone culture group and SW480 alone culture group were set up as control groups. The results found that with the increase of the proportion of colon cancer cells, the co-culture time was prolonged, the microscopic morphology of HMSC-bm cells was significantly changed, the proliferative capacity of HMSC-bm cells was enhanced, and the positive expression rate of CD34 was gradually increased; the positive rate of CD90 expression gradually cut back. This study indicates that the microenvironment of colon cancer may induce malignant transformation of HMSC-bm cells, and this malignant transformation is related to the proportion of cells and induction time.