摩腹疗法对焦虑型睡眠障碍大鼠血清NE、EPI含量的影响

Effects of abdominal therapy on serum NE, EPI contents in rats with anxiety-related sleep disorders

目的:通过检测焦虑型睡眠障碍大鼠血清中去甲肾上腺素(NE)和肾上腺素(EPI)含量,探讨摩腹疗法治疗睡眠障碍的作用机制,为临床应用提供实验依据。方法:将54只大鼠随机分为正常组(12只)、模型组(14只)、药物组(14只)、摩腹组(14只)。模型组、药物组及摩腹组采用足底电击法制作焦虑型睡眠障碍模型。造模后,正常组不做任何治疗,模型组、药物组分别给予蒸馏水、艾司唑仑溶液灌胃,摩腹组给予手法治疗,每天1次,每次约15min,10次为1个疗程,共5个疗程。治疗后采用酶联免疫法检测各组大鼠血清中NE、EPI含量。结果:模型组、药物组及摩腹组的大鼠体质量较正常组大鼠增长缓慢,差异具有统计学意义(P<0.05);大鼠血清中NE及EPI含量,模型组较正常组显著性增高(P<0.05),药物组和摩腹组较模型组显著性降低(P<0.05,P<0.01);药物组和摩腹组之间差异无统计学意义。结论:摩腹和药物治疗均有明显抗焦虑改善睡眠的作用,可能与其对NE及EPI的表达调节有关。

Objective： To investigate the mechanism of abdominal therapy for sleep disorders by detecting the noradrenaline（NE） and epinephrine（EPI） in the serum of rats with anxiety-related sleep disorders, and to provide experimental basis for clinical application. Methods： Fifty-four rats were randomly divided into normal group（12 rats）, model group（14 rats）, drug group（14 rats） and abdominal therapy group（14 rats）. Model group, drug group and abdominal therapy group used plantar shock method to establish the anxiety-related sleep disorders model. After model establishment, the normal group did not do any treatment, the model group was given distilled water, the drug group was given estazolam solution by means of intragastric administration, while the abdominal therapy group was given the manipulation therapy, once a day, 15 minutes every time, 10 times for a course of treatment, a total of 5 courses. After treatment, the levels of NE and EPI in serum of each group were measured by enzyme-linked immunosorbent assay（ELISA）. Results： The rats in the model group, drug group and abdominal therapy group had a slower increase in body weight than the normal group, and the difference was statistically significant（P〈0.05）. The contents of NE and EPI in serum of rats in the model group were significantly higher than those in the normal group（P〈0.05）, and the contents of NE and EPI in serum of rats in the drug group and the abdominal therapy group decreased than those in the model group（P〈0.05, P〈0.01）. There was no significant difference between the drug group and the abdominal therapy group. Conclusion： Both abdominal therapy and drug therapy have obvious anti-anxiety effect on improving sleep, which may be related to its regulation of NE and EPI expression. However, compared with the drug therapy, abdominal therapy is safer, more practical and worthy of promotion.