脂联素基因+45和+276多态性与川崎病的关系

Association of +45 and +276 polymorphisms in the adiponectin gene with the development of Kawasaki disease

目的检测川崎病患儿脂联素基因+45T/G、+276G/T多态性分布情况,探讨其与川崎病发病和冠状动脉损伤(CAL)发生的关系。方法采取病例对照研究方法,选取81例川崎病患儿(其中11例并发CAL)和100例正常体检儿童(对照组)。采用基因测序方法测定脂联素基因+45和+276位点的多态性分布。结果川崎病组脂联素基因+45位点基因型TT、TG、GG以及T/G的等位基因频率与对照组比较差异无统计学意义(P>0.05);川崎病组中并发CAL者与无CAL者该位点的基因型分布频率和等位基因频率比较差异亦无统计学意义(P>0.05)。川崎病组与对照组脂联素基因+276位点基因型GG、GT、TT及G/T等位基因频率的比较差异均有统计学意义(P<0.05);GG基因型为川崎病发病的危险因素(OR=2.313,P=0.006)。川崎病患儿中CAL组脂联素基因+276位点基因型分布与非CAL组比较差异无统计学意义(P>0.05)。结论脂联素基因+276位点多态性可能与川崎病的发生相关,但与CAL的发生无关;脂联素基因+45位点多态性可能与川崎病和CAL的发生均无关。

Objective To investigate the distribution of adiponectin ＋45 T/G and ＋276 G/T polymorphisms and its association with the development of Kawasaki disease and coronary artery lesion（CAL）. Methods A total of 81 children with Kawasaki disease（among whom 11 had CAL） and 100 normal children who underwent physical examination（control group） were enrolled in a case-control study. Sequencing was performed to investigate the distribution of adiponectin ＋45 T/G and ＋276 G/T polymorphisms. Results There were no significant differences between the Kawasaki disease and control groups in the frequencies of TT, TG, and GG genotypes and T/G alleles of ＋45 T/G polymorphism in the adiponectin gene（P〈0.05）. In the Kawasaki disease group, there were also no significant differences in the genotype and allele frequencies of the ＋45 T/G polymorphism between the children with CAL and those without（P〉0.05）. There were significant differences between the Kawasaki disease and control groups in the frequencies of GG, GT, and TT genotypes and G/T alleles of ＋276 G/T polymorphism in the adiponectin gene（P〈0.05）. GG genotype was a risk factor for the development of Kawasaki disease（OR=2.313, P=0.006）. In the Kawasaki disease group, there was no significant difference in the genotype distribution of the ＋276 G/T polymorphism between the children with CAL and those without（P〉0.05）. Conclusions The adiponectin ＋276 G/T polymorphism may be associated with the development of Kawasaki disease, but not associated with CAL. The adiponectin ＋45 T/G polymorphism may not be associated with Kawasaki disease or CAL.