基因组改组选育的益生性乳杆菌对小鼠肠道菌群的影响

Effect of recombinant Lactobacillus on intestinal microflora of mice

本研究利用昆明小鼠构建动物模型,通过灌胃重组菌株和野生菌株,来探讨利用基因组重组技术所选育的重组菌株对小鼠肠道菌群的影响。研究结果表明重组菌株和野生菌株均能在小鼠的小肠内定植,盲肠的数量最高,其次是结肠和回肠;激光共聚焦结果显示在小鼠空肠中粘附的重组菌株较野生菌株多;野生菌株与重组菌株均具有调节肠道菌群的作用,能降低小鼠粪便中致病菌及其他细菌的含量,且重组菌株对肠道菌群的调节能力更强。以上结果说明经基因组改组选育的菌株F3-3和F3-4由于提高了菌株的粘附能力,在小鼠肠道内形成生物占位,从而减少了致病菌和其他细菌的数量,本研究结果为进一步揭示乳杆菌发挥益生作用的分子机制提供数据支撑。

In this study, Kunming mice were used as animal models to investigate the effects of recombinant strain and wild strains on the intestinal flora of mice by gavage. The results showed that the recombinant strains and wild strains could be colonized in the small intestine of mice, and the number of cecum was the highest, followed by colon and ileum; the laser confocal microscopy results indicated that the number of recombinant strains adhered in mouse jejunum was more than that of wild strain; both wild-type and recombinant strains played a role in regulating intestinal flora and could reduce the content of pathogens and other bacteria in mice feces, and the regulation ability of recombi- nant strain was stronger. The results indicate that the genetically engineered strains F3-3 and F3-4 through increasing the adhesion ability, which formatting a biological mass in the intestinal tract of mice, reducing the number of pathogens and other bacteria. And provide data sup- port for further revealing the molecular mechanism of Lactobacillus play a beneficial role. The results further reveal the molecular mechanism of Lactobacillus to give play to probiotic function.