EV71感染SK-N-SH细胞相关基因的鉴定

Identification of Genes Involved in Enterovirus 71 Infected SK-N-SH Cells

目的:鉴定肠道病毒EV71型感染SK-N-SH细胞相关基因的改变。方法:EV71感染SK-N-SH细胞后,收集细胞做基因微阵列分析,并对差异表达的基因进行Real-time PCR和ELISA鉴定。结果:共有86个基因mRNA的转录表达改变。上调表达的基因涉及细胞因子、趋化因子、泛素蛋白以及Toll样受体信号途径、P53信号途径、凋亡途径、白细胞穿越内皮细胞、MASP信号途径和Jak-STAT信号途径等相关基因。Real-time PCR和ELISA验证了差异显著基因的高表达。结论:本研究结果提示EV71感染后的高致死率可能与一系列释放细胞因子和趋化因子的免疫反应有关,对更好地理解感染EV71病毒后机体的免疫反应提供了参考,并提供了阻断和控制EV71感染潜在的治疗策略。

Objective To explore the related alerted genes of SK-N-SH cells infected by enterovirus 71（ EV71）. Methods After SK-N-SH cells were infected with EV71,the cells were collected for gene microarray analysis,and the differentially expressed genes were identified by real-time PCR and ELISA.Results There were a total of 86 genes with altered mRNA expression. The up-regulated genes mainly involved the cytokine and chemokine,ubiquitin mediated proteolysis,Toll-like receptor signaling pathway,p53 signaling pathway,apoptosis,leukocyte transendothelial migration,MAPK signaling pathway and Jak-STAT signaling pathway. Real-time PCR and ELISA verified the high expression of differentially expressed genes.Conclusion Our results suggested that the high fatality rate of EV71 infection probably derived from a severe immune response caused by cytokines and chemokines. The findings will help to better understand the host responses to EV71 infection and provide the potential strategy for prevention and control of EV71 infection.