期刊文献+

姜黄素通过P53通路诱导人舌鳞癌细胞的凋亡 被引量:5

Curcumin Induces Apoptosis in Human Tongue Squamous Cell Carcinoma via P53 Signaling Pathway
原文传递
导出
摘要 目的:检测姜黄素对舌鳞癌细胞Tca-8113增殖和凋亡的影响,研究姜黄素对p53信号通路的影响。方法:使用浓度为10、20、40、80 mol/L的姜黄素与舌鳞癌细胞系Tca-8113共培养,在24 h和48 h后采用MTT法检测细胞的增殖情况;使用20、40 mol/L姜黄素与Tca-8113共培养24 h后,采用Western-Blot检测p53、p21和caspase9蛋白的表达。结果:姜黄素能够显著抑制Tca-8113细胞的增殖,抑制作用呈时间依赖性和药物浓度依赖性;姜黄素能够促进p53、p21和caspase 9蛋白的表达。结论:姜黄素能够抑制Tca-8113细胞的增殖并促进其凋亡,这一机制可能和p53信号通路有关。 Objective To investigate the effects of curcumin on the proliferation and apoptosis of tongue squamous carcinoma cell line Tca-8113,and to study the effect of curcumin on p53 signaling pathway. Methods The inhibition rate of cell proliferation was detected by MTT method after Tca-8113 cells were co-cultured with 10,20,40,80 μmol/L curcumin for 24 h and 48 h.The expression of p53,p21 and caspase 9 were detected by Western-Blot after Tca-8113 cells were co-cultured with 20,40 μmol/L curcumin for 24 h. Results Curcumin can significantly inhibit the proliferation of Tca-8113 cells in a time-and concentration-dependent manner. Curcumin can promote the expression of p53,p21 and caspase 9 proteins.Conclusion Curcumin can suppress the proliferation and promote apoptosis of Tca-8113 cells. This mechanism may be related to p53 signaling pathway.
作者 于曼 胡图强 YU Man;HU Tu-qiang(Department of oral medicine, Renmin People's Hospital, Hubei University of Medicine ,Shiyan, Hubei 442000, Chin)
出处 《湖北医药学院学报》 CAS 2018年第2期132-135,共4页 Journal of Hubei University of Medicine
基金 十堰市科学技术研究与开发项目计划(B20122410)
关键词 姜黄素 舌鳞癌 凋亡 p53信号通路 Curcumin Tongue Squamous Cell Carcinoma apoptosis p53 signaling pathways
  • 相关文献

参考文献1

二级参考文献10

  • 1Rebecca Siegel,Deepa Naishadham,Ahmedin Jemal.Cancer statistics, 2013[J]. CA: A Cancer Journal for Clinicians . 2013 (1)
  • 2Masashi Kanai,Atsushi Imaizumi,Yoshihiko Otsuka,Hiroki Sasaki,Momo Hashiguchi,Kazu Tsujiko,Shigemi Matsumoto,Hiroshi Ishiguro,Tsutomu Chiba.Dose-escalation and pharmacokinetic study of nanoparticle curcumin, a potential anticancer agent with improved bioavailability, in healthy human volunteers[J].Cancer Chemotherapy and Pharmacology.2012(1)
  • 3Liang-Liang Yu,Jia-Guo Wu,Ning Dai,Hong-Gang Yu,Jian-Μin Si.Curcumin reverses chemoresistance of human gastric cancer cells by downregulatingthe NF-κB transcription factor[J].Oncology Reports.2011(5)
  • 4Ratul Kumar Das,Naresh Kasoju,Utpal Bora.Encapsulation of curcumin in alginate-chitosan-pluronic composite nanoparticles for delivery to cancer cells[J].Nanomedicine: Nanotechnology Biology and Medicine.2010(1)
  • 5Jayaraj Ravindran,Sahdeo Prasad,Bharat B. Aggarwal.Curcumin and Cancer Cells: How Many Ways Can Curry Kill Tumor Cells Selectively?[J].The AAPS Journal.2009(3)
  • 6W. Glienke,L. Maute,J. Wicht,L. Bergmann.Curcumin Inhibits Constitutive STAT3 Phosphorylation in Human Pancreatic Cancer Cell lines and Downregulation of Survivin/BIRC5 Gene Expression[J].Cancer Investigation.2009(2)
  • 7Preetha Anand,Hareesh B. Nair,Bokyung Sung,Ajaikumar B. Kunnumakkara,Vivek R. Yadav,Rajeshwar R. Tekmal,Bharat B. Aggarwal.Design of curcumin-loaded PLGA nanoparticles formulation with enhanced cellular uptake, and increased bioactivity in vitro and superior bioavailability in vivo[J].Biochemical Pharmacology.2009(3)
  • 8J. Shaikh,D.D. Ankola,V. Beniwal,D. Singh,M.N.V. Ravi Kumar.Nanoparticle encapsulation improves oral bioavailability of curcumin by at least 9-fold when compared to curcumin administered with piperine as absorption enhancer[J].European Journal of Pharmaceutical Sciences.2009(3)
  • 9Mary Dillhoff,James Liu,Wendy Frankel,Carlo Croce,Mark Bloomston.MicroRNA-21 is Overexpressed in Pancreatic Cancer and a Potential Predictor of Survival[J].Journal of Gastrointestinal Surgery.2008(12)
  • 10Amit Gupta,Garima Vij,Sameer Sharma,Naveen Tirkey,Praveen Rishi,Kanwaljit Chopra.Curcumin, a polyphenolic antioxidant, attenuates chronic fatigue syndrome in murine water immersion stress model[J].Immunobiology.2008(1)

共引文献7

同被引文献35

引证文献5

二级引证文献28

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部