固定化罗伊氏乳杆菌在非严格厌氧条件下产1,3-丙二醇的研究

Study on the Production of 1,3-propanediol by Immobilized Lactobacillus reuteri under Non-strict Anaerobic Conditions

1,3-丙二醇是一种重要的平台化合物,可广泛应用于聚合物（聚酯类,聚醚类,聚氨酯类）与作为合成杂环化合物的中间体。本研究首次将固定化技术运用于罗伊氏乳杆菌产1,3-丙二醇研究,旨在探索不同固定化材料对罗伊氏乳杆菌在非严格厌氧条件下催化甘油产1,3-丙二醇的影响。研究表明,在非严格厌氧条件下,相对于海藻酸钠包埋菌体而言,采用硅藻土及斜发沸石吸附生长法固定化细胞1,3-丙二醇产量更高,产物浓度可达19.16 g/L。硅藻土固定化细胞相对于游离细胞,1,3-丙二醇产量提高35.5%,固定化细胞重复利用5次,1,3-丙二醇产量仍保持在58.04%,证明固定化效果良好。本研究以罗伊氏乳杆菌优良的菌株优势及固定化技术在非严格厌氧条件下,短时,高效生产1,3-丙二醇,为工业化大规模生产1,3-丙二醇奠定了理论基础。

1,3-propanediol is an important platform compound and can be widely used in polymers （polyesters, polyethers, urethanes） and as the intermediates of synthetic heterocyclic compounds. In this research, the immobilization technique was applied to the production of 1,3-propanediol in Lactobacillus reuteri for the first time. The aim of this study was to explore the effect of different immobilized materials on the production of 1,3-propanediol under non-strict anaerobic conditions. In non-strict anaerobic conditions, with respect to sodium alginate entrapped cells, the cells were immobilized with diatomite and clinoptilolite can obtain a higher concentration of 1,3-propanediol. The highest 1,3-propanediol concentration was 19.16 g/L. in the immobilized cells of diatomite, the yield of 1,3-propanediol was increased by 35.5% compared with that of free cells. The immobilized cells were reused 5 times and the yield of 1,3-propanediol remained at 58.04%, which proved that the immobilization effect was good. In this paper, the excellent strain of L.reuteri and the immobilization technology were used to produce 1,3-propanediol in a short time and efficiently under non-strict anaerobic conditions, which laid a theoretical foundation for the large-scale production of 1,3-propanediol.