日本蛇根草黄酮醇合成酶基因的克隆及其序列分析

Cloning and Sequence Analysis of FLS Gene in Ophiorrhiza japonica

黄酮醇合成酶（FLS）是黄酮醇生物合成途径中的重要调控酶,在黄酮醇生物合成过程中发挥着非常重要的作用。为了更好的认识日本蛇根草黄酮醇合成酶基因的结构特征,本研究以日本蛇根草为研究材料,以其转录组测序结果为基础、通过RT-PCR等方法成功克隆得到日本蛇根草FLS基因的完整c DNA序列,并采用生物信息学方法对日本蛇根草的FLS基因序列进行功能结构域、生理和化学参数、亲/疏水性、信号肽、二级结构等预测分析。分析结果表明,该基因序列全长1 008 bp,编码335个氨基酸,预测其蛋白分子量为38.342 k D,等电点为5.87,为亲水性蛋白质,不含有信号肽。

Flavonol synthase （FLS） is a key regulatory enzyme in the flavonol biosynthetic pathway, which plays an important role during flavonol biosynthesis. For a better understanding of the structural characteristics of flavonol synthase （FLS） gene in Ophiorrhiza japonica, taking Ophiorrhiza japonica as materials, we successfully cloned the integrated cDNA sequence of FLS in Ophiorrhiza japonica based on the result of its transcriptome sequencing through RT-PCR method. The parameters of FLS gene sequence from Ophiorrhiza japonica, including functional domains, physical and chemical parameters, hydrophobicity or hydrophilicity, signal peptide, and secondary structure, were analyzed and predicted by using bioinformatics method. The bioinformatics analysis results showed that the full-length of FLS was 1 008 bp, encoding 335 38.342 kD and its isoelectric points were 6.05. FLS of signal peptide. amino acids. Its putative protein molecular weight was Ophiorrhiza japonica was a hydrophilic protein without