‘脆红李’果实发育过程中花色苷含量及相关基因表达分析

Analyses of Anthocyanin Accumulation and Expression Patterns of Anthocyanin Synthesis Genes during the Developmental Stages in’Cuihongli’

花色苷是一类重要的色素,对李红色的形成必不可少。本研究以‘脆红李’为试材,研究了果实发育过程中叶绿素含量、总花色苷含量及果皮主要花色苷组分和含量的变化规律,并分析了Ps PAL、Ps CHS、Ps CHI、Ps F3H、Ps DFR、Ps ANS和Ps UFGT基因在果实不同发育阶段的表达规律。结果表明,随着‘脆红李’果实的生长发育,果皮和果肉中总叶绿素含量呈逐渐下降的趋势;‘脆红李’果肉中不含花色苷,果皮中的花色苷在转色期才开始积累,成熟时达到最大值,为404.37μg/（g·FW）,并以矢车菊素-3-O-葡萄糖苷和矢车菊素-3-O-芸香糖苷为主;花色苷合成相关基因在‘脆红李’不同生长发育时期的果皮和果肉中有着特异性的表达,但只有Ps PAL和Ps UFGT基因的转录水平与花色苷含量的正相关性达到极显著水平,表明这两个基因对‘脆红李’果实的着色有着异常重要的调控作用。

Anthocyanins are important pigments and are responsible for red coloration in plums. The plum ＇Cuihongli＇ was used to study the changes of the contents of chlorophyll and anthocyanin and the transcription of the genes encoding seven enzymes （ PsPAL, PsCHS, PsCHI, PsF3H, PsDFR, PsANS, PsUFGT） during the developmental stages. The results showed that the content of total chlorophyll in the peel and flesh decreased gradually with the development of ＇Cuihongli＇; The anthocyanin content in the peel began to accumulate in the color-changed period, and reached the maximum value at maturity, which was 404.37 μg/（g ·FW）, and the main components were cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside. Anthocyanin synthesis related genes had specific expression in the peel and flesh of ＇Cuihongli＇ at different growth stages. However, only the positive correlation between the transcription level of PAPAL and PsUFGT gene and anthocyanin content reached a very significant level, indicating that these two genes had an important role in the regulation of the appearance of ＇Cuihongli＇.