未成熟树突状细胞与成熟树突状细胞的细胞骨架调控相关基因表达的差异分析

Differential Analysis on Gene Expression Related to Cytoskeleton Regulation between Immature Dendritic Cells and Mature Dendritic Cells

分析未成熟树突状细胞（immature dendritic cells,im DCs）向成熟的树突状细胞（mature dendritic cells,m DCs）分化的过程中,细胞骨架的调控相关基因及信号通路的表达变化,为进一步理解不同分化阶段树突状细胞（dendritic cells,DCs）的生物物理学特性、迁移能力和免疫学相关功能的改变。利用CEO数据库获得经CD14^＋单核细胞（monocytos,monos）诱导而成的im DCs和m DCs的m RNA的表达数据（芯片编号：GSE15076）,通过R语言软件对原始数据进行处理,筛选差异表达基因（Log｜FC｜≥2,p〈0.05）,利用STRING online-工具对差异表达基因进一步筛选（可信度≥0.4）;Cytoscape软件构建蛋白质相互作用网络图（protein-protein interaction network,PPI）,通过Cluster ONE对筛选后的差异表达基因进行聚类分析,筛选功能相关性密切的差异表达基因,并利用DAVID在线分析进一步进行GO分析和KEGG信号通路分析。m DCs相对于im DCs差异表达的基因共3 351个,上调基因1 801个,下调基因1 550个,其中C-C趋化因子受体活性、G-蛋白偶联的嘌呤核苷酸受体活性和异源三聚体G蛋白复合物等与细胞骨架调控密切相关。主要涉及的信号通路包括趋化因子/趋化因子受体信号通路、Rap1信号通路、Jak-STAT信号通路和PI3K-Akt信号通路等,其中的相关基因与细胞骨架的调控关系密切。这对进一步深入理解DCs的生物物理学特性、迁移能力和免疫学功能有一定的帮助。

To further understand the different biophysical characteristics, motilities and immune functions of DCs at different differentiation stagess by bioinformatics analysis of the cytoskeleton-associated different-expressed genes, mRNA expression of immature dendritic cells （imDfs） and mature DCs （mDCs） which induced by monocytos were obtained from the CEO database Data （chip number： GSE15076）, The differentially expressed genes were screened by R language software （Log｜FC｜ ≥ 2, p 〈0.05）, and the differentially expressed genes were further screened by STRING online-tool, Cytoscape software was used to construct a protein-protein interaction network （PPI）. Clustered analysis of the differentially expressed genes was performed by ClusterONE. The analysis of GO and KEGG signaling pathway of differentially expressed genes were screened and analyzed by DAVID online. There were 3 351 genes with different expression between mDCs and imDCs, among which the number of up-regulated genes was 1 801 and the down-regulated was 1 550, in which that C-C chemokine receptor activity, G-protein-coupled purine nucleotide receptor activity and heterotrimer G protein complex were closely related to cytoskeleton regulation. The main signaling pathways include chemokine/chemokine receptor signaling pathway, Rap l signal pathway, Jak-STAT signal pathway and PI3K-Akt signaling pathway. The related genes were closely related to the regulation of cytoskeleton. This provides a basis for further understanding of the biophysical characteristics, motilities and immunological functions of DCs.