摘要
比对分析由双核菌丝L808制备的两个原生质体单核菌丝L808P65和L808P78与双核菌丝L808之间的内切-β-1,4-葡聚糖酶(Cx)活性和IGS1序列差异。结果表明:L808双核菌丝生长速度快,分泌胞外酶快且含量高,Cx比活居中,拥有两个长度不同的IGS1序列:L808-c1(1003 nt)和L808-c2(1041 nt);L808P65单核菌丝生长速度居中,分泌胞外酶含量和速度居中,Cx比活最低,拥有与L808-c1一样的IGS1序列;L808P78单核菌丝生长速度最慢,分泌胞外酶含量和速度最小,但Cx比活最高,拥有与L808-c2一样的IGS1序列。
Enzyme activity of endo-β-1,4-glucanases（Cx） and IGS1 polymorphism difference among twoprotoplasted monokaryotic mycelia,L808 P65 and L808 P78（isolated from dikayotic mycelia L808）,and dikayoticmycelia L808 were studied in the paper. The results showed that：dikayotic mycelia L808 grew fastest,secretedthe highest extracellular enzyme content in the fastest speed,had the higher Cx activity,and owned two IGS1 sequences with different length,L808-c1（1003 nt）and L808-c2（1041 nt）; monokaryotic mycelia L808 P65 grewfaster,secreted the higher extracellular enzyme content in the faster speed,had the low enzyme activity and itsIGS1 sequence was identical with L808-c1; monokaryotic mycelia L808 P78 grew slowly, secreted the lowextracellular enzyme content in the slow speed, had the highest enzyme activity, and its IGS1 sequence wasidentical with L808-c2.
作者
宋晓霞
王倩
赵妍
黄建春
宋春艳
陈明杰
谭琦
SONG Xiao-xia;WANG Qian;ZHAO Yan;HUANG Jian-chun;SONG Chun-yan;CHEN Ming-jie;TAN Qi(Institute of Edible Fungi ,Shanghai Academy of Agricultural Science;Key Laboratory of Edible Fungi Resources and Utilization （ South）, Ministry of Agriculture, P. R. Chin;National Engineering Research Center of Edible Fung;National R＆D Center for Edible Fungi Processin;Key Laboratory of Agricultural Genetics and Breeding of Shanghai ,Shanghai 201403, Chin)
出处
《上海农业学报》
CSCD
2018年第3期54-58,共5页
Acta Agriculturae Shanghai
基金
上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-13号]
国家食用菌产业技术体系资助项目(CARS-20)
关键词
香菇
单核菌丝
双核菌丝
互作
多态性
Lentinula edodes
Monokaryotic mycelium
Dikayotic mycelium
Interaction
Polymorphism
