摘要
比对分析由双核菌丝L808制备的两个原生质体单核菌丝L808P65和L808P78与双核菌丝L808之间的内切-β-1,4-葡聚糖酶(Cx)活性和IGS1序列差异。结果表明:L808双核菌丝生长速度快,分泌胞外酶快且含量高,Cx比活居中,拥有两个长度不同的IGS1序列:L808-c1(1003 nt)和L808-c2(1041 nt);L808P65单核菌丝生长速度居中,分泌胞外酶含量和速度居中,Cx比活最低,拥有与L808-c1一样的IGS1序列;L808P78单核菌丝生长速度最慢,分泌胞外酶含量和速度最小,但Cx比活最高,拥有与L808-c2一样的IGS1序列。
Enzyme activity of endo-β-1,4-glucanases(Cx) and IGS1 polymorphism difference among twoprotoplasted monokaryotic mycelia,L808 P65 and L808 P78(isolated from dikayotic mycelia L808),and dikayoticmycelia L808 were studied in the paper. The results showed that:dikayotic mycelia L808 grew fastest,secretedthe highest extracellular enzyme content in the fastest speed,had the higher Cx activity,and owned two IGS1 sequences with different length,L808-c1(1003 nt)and L808-c2(1041 nt); monokaryotic mycelia L808 P65 grewfaster,secreted the higher extracellular enzyme content in the faster speed,had the low enzyme activity and itsIGS1 sequence was identical with L808-c1; monokaryotic mycelia L808 P78 grew slowly, secreted the lowextracellular enzyme content in the slow speed, had the highest enzyme activity, and its IGS1 sequence wasidentical with L808-c2.
作者
宋晓霞
王倩
赵妍
黄建春
宋春艳
陈明杰
谭琦
SONG Xiao-xia;WANG Qian;ZHAO Yan;HUANG Jian-chun;SONG Chun-yan;CHEN Ming-jie;TAN Qi(Institute of Edible Fungi ,Shanghai Academy of Agricultural Science;Key Laboratory of Edible Fungi Resources and Utilization ( South), Ministry of Agriculture, P. R. Chin;National Engineering Research Center of Edible Fung;National R&D Center for Edible Fungi Processin;Key Laboratory of Agricultural Genetics and Breeding of Shanghai ,Shanghai 201403, Chin)
出处
《上海农业学报》
CSCD
2018年第3期54-58,共5页
Acta Agriculturae Shanghai
基金
上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-13号]
国家食用菌产业技术体系资助项目(CARS-20)
关键词
香菇
单核菌丝
双核菌丝
互作
多态性
Lentinula edodes
Monokaryotic mycelium
Dikayotic mycelium
Interaction
Polymorphism