基于Illumina MiSeq高通量测序分析黄芪内生细菌多样性

Diversity of endophytic bacteria in Astragali Radix analyzed by Illumina MiSeq high-throughput sequencing technology

目的分析黄芪内生细菌群落多样性,为筛选菌种发酵转化黄芪中活性成分的定向炮制工艺研究提供参考依据。方法应用Illumina Mi Seq高通量测序技术测定黄芪内生细菌的16 S rDNA V3-V4区域序列并进行物种丰度等生物信息分析。结果获得有效序列40 051条,操作分类单元(OTUs)967个,测序数量接近于饱和,测序数据量合理。黄芪内生细菌主要分布于Anderseniella属、芽孢杆菌属Bacillus、伯克氏菌属Burkholderia、不动杆菌属Acinetobacter、寡养单胞菌属Stenotrophomonas、海洋芽胞杆菌属Oceanobacillus 6个属。结论黄芪内生细菌群落多样性较低,Anderseniella属和芽孢杆菌属Bacillus是黄芪内生细菌的优势种群。

Objective This study aimed to analyze the diversity of endophytic bacterial communities, and to provide reference to the oriented processing technology, which helps to screen bacteria fermentation and conversion in the active ingredients of Astragali Radix. Methods The 16 S rDNA V3-V4 region of Astragali Radix was sequenced by Illumina Mi Seq high-throughput sequencing technology, and the abundance of species and other biological information were analyzed. Results The results showed that the numbers of effective sequences and OTUs for sample were 40 051 and 967, respectively. The number of sequencing was close to saturation, and the sequencing data volume was reasonable. The endophytic bacteria of Astragali Radix mainly belonged to Anderseniella, Bacillus, Burkholderia, Acinetobacter, Stenotrophomonas, and Oceanobacillus. Conclusion The diversity of endophytic bacteria was low in Astragali Radix. The dominant population of endophytic bacteria in Astragali Radix belongs to Anderseniella and Bacillus.