人纤维蛋白黏合剂在新西兰兔体内的组织分布与排泄

Tissue distribution and excretion of fibrin sealant in New Zealand rabbits

目的研究主要由纤维蛋白原和凝血酶组成的人纤维蛋白黏合剂(FS)在新西兰兔体内的组织分布及排泄特点。方法实验中使用的FS组方含有放射性^(125)I的凝血酶。凝血酶用Iodogen方法进行^(125)I标记凝血酶,采用肝局部给药方式,结合分子筛高效液相色谱法(SHPLC)及γ-计数法测定不同时间组织及体液样品中^(125)I-凝血酶的含量。结果放射性物质主要存留在肝给药部位,其次是血浆,在其他组织只有痕量分布;^(125)I-凝血酶给药后8 d内经尿累积排泄率为(91.54±9.65)%,经粪便累积排泄率为(3.42±0.34)%。结论肝局部给药后,FS进入血浆,主要经肾排泄,8 d后排泄完全。研究结果为FS更为合理的临床实际应用提供了参考依据。

Objective To investigate the tissue distribution and excretion of fibrin sealant（FS）that is mainly composed of fibrinogen and thrombin in New Zealand rabbits. Methods The experiment was performed using the FS formulation with 125I-radiolabled thrombin. Thrombin was labeled with 125I by the Iodogen method. After liver-local administration of FS,the concentration of 125Ithrombin in various tissues and body fluids was determined by size-exclusion high performance liquid chromatography（SHPLC）combined with γ-counting methods. Results After the liver-local administration of FS,the 125I-thrombin predominantely accumulated in the injection site and,with a few in other tissues. The cumulative excretion rate of 125I-thrombin was（91.54±9.65）% in urine and（3.42±0.34）% in feces within 8 days of administration. Conclusion FS could be absorbed into the blood after the liver-local administration,then be underwent the major renal excretion and was almost completely eliminated within 8 days. The present results provide a reference for the more rational clinical application of FS.