以壳聚糖为絮凝剂的杜仲叶水提液澄清工艺优化

Optimization technology of the clarification process for the water-extracted solution of Folium Eucommiae with chitosan flocculant

目的优化杜仲叶水提液的絮凝除杂工艺,保留有效组分的同时提高原药水提液的澄清度。方法采用壳聚糖絮凝法,在保证药液澄清的前提下,以水提液中绿原酸、芦丁、槲皮素和山奈酚的保留率为考察指标,用单因素实验初步确定水提液的浓缩比、絮凝温度和壳聚糖用量3个因素的适宜范围,并运用正交实验综合优选最佳澄清工艺。结果壳聚糖作为絮凝剂用于杜仲叶水提液的最佳絮凝工艺条件为药材(g)-药液浓缩比(L)为30∶1、絮凝温度40℃、壳聚糖加入量0.7 g/L。在此条件下,溶液的澄清度好,上述4种成分的保留率依次为94.3%、83.1%、80.1%和85.4%。结论壳聚糖絮凝法纯化杜仲叶水提液的工艺简单易行,能保证药效组分较高的保留率同时提高了药液的澄清度和稳定性。

Objective To optimize the clarification process of water-extracted solution（WES）of Folium Eucommiae（FE）so as to reserve more amounts of bioactive components and improve the clarity. Methods Chitosan was used as flocculant for the clarification of FE-WES,and the reservation percentage of chlorogenic acid,rutin,quercetin and kaempferol was set as an index for estimating the reservation of bioactive components. The related parameters for the flocculation, including the flocculant dosage,flocculation temperature and concentrated ratio of FE-WES to the raw material FE,were optimized by the orthogonal design method. Results The optimal conditions for flocculations were as follows：the FE（g）-WES（L）ratio was 30∶1,the flocculation temperature was 40℃,and the percentage of added chitosan was 0.7 g/L. Under these conditions,WES showed best clarity, with the reservation percentage of94.3%,83.1%,80.1% and 85.4% for chlorogenic acid, rutin, quercetin and kaempferol,respectively. Conclusion The present flocculation process is simple and practical,which may ensure a higher reservation of bioactive components and effectively enhance the clarity and stability of FE-WES.