摘要
目的了解河北省2014-2016年侵袭性肺炎链球菌（Streptococcus pneumonia,Sp）的血清型以及多位点序列分型（Multilocus sequence type,MLST）分布。方法收集河北省自2014-2016年从14岁以下Sp疾病儿童中分离的侵袭性Sp菌株,应用聚合酶链式反应进行血清型鉴定和MLST测序分型。结果共分离到88株侵袭性Sp,主要血清型为19F（17株,9.3%）、14（16株,18.2%）、6A/6B（10株,11.4%）、19A（9株,10.2%）、20（6株,6.8%）、1（5株,5.7%）和23F（5株,5.7%）,这7种血清型共占77.3%;其余血清型包括6C/6D（4株）、12F/12A/44/46（3株）、3（2株）、5（2株）、7F/7A（1株）、9V/9A（1株）,共占14.8%;7株（7.9%）未能分血清型。在所有侵袭性Sp中检出38种序列分型（ST）,主要为ST-271（11株,12.5%）、ST-320（8株,9.1%）、ST-876（5株,5.7%）、ST-2248（5株,5.7%）、ST-2296（5株,5.7%）。结论河北省2014-2016年侵袭性Sp血清型以19F、14、6A/6B、19A为主;ST以ST-271、ST-320为主。
Objective To determine the distribution of serotypes and multi-locus sequence types（ MLST）of invasive Streptococcus pneumonia（ Sp） isolated from 2014 to 2016 in Hebei province. Methods We collected Sp strains isolated from children under 14 years old with invasive Sp disease in Hebei during2014-2016. We used polymerase chain reaction to identify serotypes and MLST sequence types of Sp strains. Results A total of 88 Sp strains were isolated,among which the dominant serotypes were 19 F（ 17 strains,19. 3%）,14（ 16 strains,18. 2%）,6 A/6 B（ 10 strains,11. 4%）,19 A（ 9 strains,10. 2%）,20（ 6 strains,6. 8%）,1（ 5 strains,5. 7%）,and 23 F（ 5 strains,5. 7%）; these 7 serotypes accounted for 77. 3% of the isolates. Other isolated serotypes included 6 C/6 D（ 4 strains）,12 F/12 A/44/46（ 3 strains）,3（ 2 strains）,5（ 2 strains）,7 F/7 A（ 1 strain）,and 9 V/9 A（ 1 strain）; these6 serotypes accounted for 14. 8% of the isolates. Seven isolates could not be identified by serotype. Thirty-eight Sp strains were identified as sequence types（ STs）,in which the dominant STs were ST-271（ 11 strains,12. 5%）,ST-320（ 8 strains,9. 1%）,ST-876（ 5 strains,5. 7%）,ST-2248（ 5 strains,5. 7%）,and ST-2296（ 5 strains,5. 7%）. Conclusions The dominant invasive Sp strains from 2014 to2016 in Hebei were 19 F,14,6 A/6 B,and 19 A in serotypes,or ST-271 and ST-320 in STs.
作者
王乐雨
贾肇一
刘殿武
王颖童
何宝花
王婧
朱兵清
邵祝军
张永茂
孙印旗
Wang Leyu;Jia Zhaoyi;Liu Dianwu;Wang Yingtong;He Baohua;Wang Jing;Zhu Bingqing;Shao Zhujun;Zhang Yongmao;Sun Yinqi(Hebei Medical University, Shijiazhuang 050000;Hebei, Chin;2. Hebei Provincial Center for Disease control and Prevention, Shift- azhuang 05000;Hebei, Chin;3. Bethune International Peace Hospital, Sh~iiazhuang 050000, Hebei, China;4. Institute for Communicable Disease Prevention anf Control, Chinese Center for Disease Control and Prevention, Bering 102206, China)
出处
《中国疫苗和免疫》
北大核心
2018年第3期289-293,284,共6页
Chinese Journal of Vaccines and Immunization
基金
河北省科技计划项目课题(16277745D)
关键词
侵袭性肺炎链球菌
血清型
多位点序列分型
Invasive Streptococcus pneumoniae
Serotype
Multi-locus sequence type
