薯蓣皂苷元体外作用对胶原诱导性关节炎小鼠Th1/Th2型细胞因子的影响

Effect of diosgenin on type of Th1/Th2 cytokines in CIA mice in vitro

目的探讨薯蓣皂苷元体外作用对胶原诱导性关节炎(CIA)小鼠外周淋巴细胞分泌细胞因子水平的影响。方法建立CIA小鼠模型,无菌取外周淋巴组织,提取淋巴细胞体外培养,将淋巴细胞分为空白组、细胞模型组、高、中、低剂量组,采用基因芯片法检测各组培养上清中辅助性T细胞(Th)1/Th2型细胞因子γ-干扰素(IFN-γ)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-4、IL-5的表达水平。结果薯蓣皂苷元作用后IFN-γ表达水平有降低趋势,其中高剂量组薯蓣皂苷元对IFN-γ表达有显著的抑制作用(P<0.01)。经薯蓣皂苷元作用后TNF-α有降低趋势,但与模型组比较差异并无统计学意义(P>0.05)。经薯蓣皂苷元作用后IL-4水平有上升趋势,其中中、高剂量薯蓣皂苷元可以显著提高IL-4水平(P<0.05)。低、中、高剂量薯蓣皂苷元作用后可以显著提高IL-5水平(P<0.01,P<0.05)。结论薯蓣皂苷元体外对CIA小鼠外周淋巴细胞Th1/Th2型细胞因子具有一定的调节作用,可能与其治疗类风湿关节炎有关。

Objective To investigate the immune regulation mechanism of diosgenin in rheumatoid arthritis,the Th1/Th2 type cytokines were detected in culture supernatant of lymphocytes from CIA mice after application of diosgenin in vitro. Methods After CIA mice models were established,the peripheral lymphoid tissues were separated with aseptic and the lymphocytes were extracted and cultured in vitro. The lymphocytes were divided into control,cell model,high,middle,low drug groups. The levels of IFN-γ,TNF-α,IL-4 IL-5 of Th1/Th2 type cytokines were examined by gene chip.Results The level of IFN-γ was decreased,especially in high dose group of diosgenin,the level of IFN-γ was significantly inhibited（ P〈0.01）. The level of TNF-αwas decreased,but there was no significant difference compared with that of cell model group.The level of IL-4 had a rising trend; the middle dose,high dose of diosgenin significantly elevated the level of IL-4（ P〈0.05）. Compared with that of cell model group,the level of IL-5 was significantly higher in low,middle,high dose of diosgenin groups（ P〈0.01,P〈0.05）.Conclusions Diosgenin has a regulatory role on Th1/Th2 type cytokines of CIA mice peripheral lymphoid cells.