HA/GCPU复合多孔支架的可控制备及细胞相容性

Preparation and Cytocompatibility of Hydroxyapatite/glyceride Based Polyurethane Porous Scaffolds

以脂肪族异佛尔酮二异氰酸酯(IPDI)作为硬段、蓖麻油甘油酯(GCO)作为软段,通过原位聚合法制备了羟基磷灰石/蓖麻油甘油酯基聚氨酯(HA/GCPU)复合多孔支架.利用红外光谱和扫描电子显微镜对复合支架进行表征,并测试其力学性能及孔隙率.研究结果表明,HA/GCPU复合多孔支架的孔隙率和抗压强度依赖羟基磷灰石的含量,并具有明显的可控性.HA质量分数分别为0,20%和40%时,HA/GCPU复合多孔支架孔隙率分别为(61±3)%,(68±2)%和(57±3)%,抗压强度分别为(605±61),(2125±58)和(4588±260)k Pa,可见HA质量分数为20%时,HA/GCPU复合多孔支架具有与松质骨较为匹配的孔隙率和抗压强度.将MG63细胞与多孔支架共培养,通过倒置显微镜和扫描电子显微镜观察,用噻唑蓝(MTT)法表征HA/GCPU复合多孔支架的细胞相容性,结果表明,HA/GCPU复合多孔支架表面细胞黏附,生长良好,无细胞毒性,在骨组织工程领域具有一定的应用前景.

Castor oil was transesterified with glycerol,the glyceride of castor oil（ GCO） copolymerized with isophorone diisocyanate（ IPDI） to generate polyurethane（ PU）. The porous scaffolds of hydroxyapatite/glyceride based polyurethae（ HA/GCPU） were prepared by in situ polymerization. The GCPU and HA/GCPU porous scaffolds were characterized by Fourier transforw infrared spectroscopy（ FTIR） and scanning electron microscopy（ SEM）. The mechanical property and porosity were tested. The results showed that the porosity of GCPU,HA/GCPU composite with 20% HA and HA/GCPU composite with 40% HA were（ 61 ± 3） %,（ 68±2） % and（ 57 ± 3） %,respectively. The compressive strength of above three porous scaffolds were605±61,2125± 58 and（ 4588 ± 260） k Pa,respectively. To further evaluate the cytocompatibility of porous scaffolds,the MG63 cells were co-cultured on scaffolds,and light microscopy observation,SEM observation,3-（ 4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium-bromide（ MTT） assay were carried out. The results demonstrated that both pure GCPU and HA/GCPU scaffolds had no cytotoxicity. It is believed that HA/GCPU composite porous scaffolds have the potential to be applied in bone regeneration.