摘要
利用硅胶柱色谱、凝胶柱、反向柱色谱和高效液相等手段对海绵来源的真菌Alternaria sp.F49在大米培养基中的代谢产物进行分离纯化,从中分离得到一个新的聚酮类化合物:3R,5R-(-)-talaroflavone(2)和15个已知化合物。Ⅰ及Ⅱ是两个消旋体,通过手性柱分别将它们进行拆分得到1和2、3和4两对对应异构体;通过核磁、质谱和文献比对确定了这些化合物的结构,运用X-单晶衍射及圆二色谱等手段确定了化合物1、2的绝对构型;化合物6、8~10首次从该属真菌(Alternaria)中分离出来;化合物16展示出中等的COX-2酶抑制活性,其IC_(50)为7.3μmol·L^(-1)。
A new polyketide: 3R, 5R-(-)-talaroflavone(2), along with 15 known compounds were isolated from a EtOAc extract of a sponge-derived fungus Alternaria sp. F49. Compounds 1-2 and 3-4 were separated as two pairs of enantiomers by chiral HPLC from Ⅰ and Ⅱ. The structures of compounds 1-16 were elucidated by means of NMR and MS. Furthermore, the absolute configurations of compounds 1-2 were determined by single crystal X-ray diffraction experiment and CD analyses. Compounds 6, 8-10 were isolated from this genus(Alternaria sp.) for the first time. Compound 16 showed moderate COX-2 enzyme inhibitory activity with IC50 of 7.3 μmol·L^-1.
作者
田永奇
林舒婷
刘永宏
TIAN Yong-qi;LIN Shu-ting;LIU Yong-hong(College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China;Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China)
出处
《药学学报》
CAS
CSCD
北大核心
2018年第7期1134-1138,共5页
Acta Pharmaceutica Sinica
基金
福州大学科研启动基金(XRC-1672)
福州市科技局项目(2017-N-39)
福建省教育厅项目(JAT170077)
广东省海洋药物重点实验室(LMM)2018年度开放课题(2018011009)