基于电诱导沉积原理制备形状可控的海藻酸钙-多聚赖氨酸水凝胶微封装胶囊

Shape-Controlled Fabrication of Calcium Alginate-PLL Hydrogel Microcapsules Based on Electrodeposition Method

海藻酸钙水凝胶是一种具有良好生物相容性、生物降解性的生物医用高分子材料。文中提出了一种基于电诱导沉积原理制备形状可控的海藻酸钙-多聚赖氨酸(PLL)水凝胶微封装胶囊。在涂有光刻胶的FTO导电玻璃表面上利用光刻技术制造多种预设图案的微电极,基于电诱导沉积原理在微电极上制备特定几何结构海藻酸钙水凝胶,通过PLL等试剂的处理最终得到环形与六边形结构的海藻酸钙-PLL水凝胶微封装胶囊,酵母细胞包含在微封装胶囊中进行24 h的培养,获得有一定生物活性的细胞。这种方法可以制备用于组织工程学研究的生物支架,将对细胞装配,生物打印以及药物输送等领域有重要的参考价值。

Calcium alginate hydrogel is a kind of well biocompatible and biodegradable biomedical polymer material.However,hydrogel with physical structure cannot be obtained by traditional preparation method. This paper proposed an electrodeposition method of fabricating shape-controlled calcium alginate-poly-L-lysine（ PLL） hydrogel microcapsules.The micro-patterned electrodes,which were produced by coating a patterned photoresist layer onto FTO glass slide based on photolithography technique,were used for making different shapes of alginate hydrogel structures. Then,the structures would be detached from the electrode surface and treated with given reagent to be transformed into 3 D microcapsules while maintaining the ring and hexagon shapes. Finally,the yeast cells within microcapsules can maintain certain bioactivity after 24 h＇cultivation. The recommended method can lead to fabricating cell-laden scaffold for tissue engineering. It will be instructive in the field of cells assembly,bio-printing and drug discovery.