辛醇辅助磁固相萃取-HPLC检测环境水中酚类物质

Octanol enhanced MSPE coupled with HPLC for the determination of five phenolic compounds in environmental water

建立了一种磁固相萃取(MSPE)-高效液相色谱法(HPLC)测定环境水样中的5种酚类化合物(双酚A、双酚AF、四溴双酚A、辛基酚和壬基酚)的方法.利用磁性纳米材料易分散、分离速度快的特点,以辛醇和Fe3O4@壬酸磁性纳米材料为萃取剂,辛醇通过涡旋混合吸附在磁性材料上,构成Fe3O4@壬酸@辛醇体系,实现对目标物的高效、快速吸附.实验考察了影响目标物萃取效率的条件,在最佳条件下,5种酚类化合物的线性范围为0.5~100.0μg·m L^(-1)(R2>0.998),检出限(LODs)范围为0.07~0.16μg·m L^(-1)(S/N=3),样品的加标回收率在88.7%~98.2%,相对标准偏差(RSDs,n=5)范围为2.9%~5.9%.

A simple and effective method based on octanol enhanced magnetic solid- phase extraction （MSPE） coupled with high-performance liquid chromatography （HPLC） for the determination of five phenolic compounds （ BPA, BPAF, TBBPA, NP and OP） in environmental water was established. Using the characteristics of magnetic nanoparticles which are easy to disperse and fast to separate, octanol and Fe304@ n-nonanoic acid nanoparticles were used as extractant, furthermore, octanol was used to coat n-nonanoic acid Fe3O4 nanoparticles with core-shell structures to prepare MSPE agents （Fe3O4 @ n-nonanoic acid @ octanol）.The main parameters affecting the extraction efficiency of five phenolic compounds were studied. Under optimal conditions, the calibra- tion curves were linear in the range of 0.5--100μg · mL-1（R2〉0.998） of five phenolic compounds.The limit of detections （LODs） and relative standard deviations （ RSDs, n = 5 ） of the method were 0.07--0.16 μg · mL-1 and 2.9%--5.9% ,respectively.The adding standard recoveny of the sanple is 88.7%--98.2%.