红托竹荪对小鼠IL-12、IL-4和IFN-γ表达影响

Influence of Dictyophora rubrovolvata on IL-12,IL-4 and IFN-γ expression in mice

目的探讨红托竹荪对小鼠免疫功能的影响。方法用不同浓度红托竹荪按0.02mL/g对小鼠连续灌胃30 d,在第20天开始对环磷酰胺组、红托竹荪低、中、高剂量组(5、10、15 g/kg)小鼠腹腔注射50 mg/kg环磷酰胺,连续3d,同时各剂量红托竹荪组继续进行红托竹荪灌胃。禁食24h后称重,小鼠眼球取血,分离脾脏和胸腺。测定小鼠血清中白细胞介素-12(IL-12)、IL-4和γ-干扰素(IFN-γ)水平。结果与对照组比较,环磷酰胺组小鼠体重、脾脏指数、胸腺指数无明显变化;与环磷酰胺组比较,高剂量红托竹荪组小鼠体重、脾脏指数、胸腺指数明显升高(P<0.05)。与对照组比较,环磷酰胺组小鼠血清中IL-12、IL-4和IFN-γ含量[分别为(18.12±3.55)、(10.19±2.29)、(12.73±2.79)ng/mL]均明显下降,差异有统计学意义(P<0.05);与环磷酰胺组比较,中、高剂量红托竹荪组小鼠血清中IL-12、IL-4和IFN-γ含量[分别为(32.58±3.95)、(17.81±4.23)、(22.58±3.76)和(31.73±4.30)、(23.50±1.66)、(25.27±3.86)ng/mL]均升高,差异有统计学意义(P<0.05)。结论红托竹荪可以促进IL-12、IL-4和IFN-γ表达,对环磷酰胺诱导的小鼠免疫功能损伤具有一定保护作用。

Objective To explore the effects of Dictyophora rubrovolvata on immune function in mice. Methods Forty female Kunming mice were randomly assigned into a control and a cyclophosphamide （Cy） group gavaged with distilled water and low-, moderate-, and high-dose groups gavaged with water extraction of Dictyophora rubrovolvata at dosages of 5, 10, 15 g/kg continuously for 30 days. At the 20th day of the gavage, all the mice were administered with Cy by intraperitoneal injection at the dosage of 50 mg/kg·body weight once a day continuously for 3 days, except for the mice of control group. At the end of treatment, all the mice were weighted and their blood samples were collected and their spleens and thymuses were separated after a 24-hour fasting. Serum interleukin 12 （IL-12）, interleukin 4 （IL-4）, and interferon gamma （IFN-γ） of the mice were detected. Results No significant difference in body weight, spleen index, and thymus index were observed between the mice of control and Cy group; while the body weight, spleen and thymus index of mice of high Dictyophora rubrovolvata group increased significantly compared to those of the Cy group （P 〈 0.05 for all）. Serum IL-12 （18.12±3.55 ng/ml）, IL-4 （18.12±3.55 ng/ml）, and IFN-γ （12.73±2.79 ng/ml） of the Cy group decreased significantly compared to those of the control group （P 〈 0.05 for all）. In comparison with those of the Cy group, significantly increased serum IL-12 （32.58±3.95 and 31.73±4.30 ng/ml）, IL-4 （17.81±4.23 and 23.50±1.66 ng/ml）, and IFN-γ （22.58±3.76 and 25.27±3.86 ng/ml） were detected in the moderate- and high-dose Dictyophora rubrovolvata groups （all P 〈 0.05）. Conclusion Dictyophora rubrovolvata can promote expressions of IL-12, IL-4, and IFN-γ and antagonize adverse impact of cyclophosphamide on immune function in mice.