牛蒡根提取物BP1对Ⅱ型糖尿病大鼠症状的改善作用

Therapeutic Effect of Burdock Root Extract BP1 on the Type Ⅱ Diabetic Rats

为了研究牛蒡根提取物BP1对糖尿病的治疗作用,本文分别对wistar糖尿病模型大鼠灌喂阿卡波糖200 mg/kg、生理盐水0.9%、牛蒡根提取物BP1 400 mg/kg、600 mg/kg及800 mg/kg处理,每周监测各组大鼠的死亡情况、体重及空腹血糖,9周后解剖大鼠,测血液生化指标;肾脏胰腺部分组织进行石蜡包埋并HE染色,另外取肾脏部分组织于荧光定量PCR使用。结果表明,牛蒡根提取物BP1可在一定程度上恢复糖尿病大鼠的体重,调节大鼠血清血脂,降低糖尿病鼠空腹血糖;电镜观察牛蒡根提取物BP1可以显著增加胰岛β细胞中胰岛素分泌颗粒;另外,BP1可促进IL-18、TNF-α的表达水平明显下调,降低了糖尿病大鼠的肾脏细胞损伤情况;并通过提高SOD酶活性改善糖尿病鼠肾脏及胰腺的氧化损伤情况,对肾脏胰腺器官具有一定保护作用。

In order to study the therapeutic effect of burdock root extract BP1 on the diabetic rats, the wistar diabetic rats were fed with acarbose 200 mg/kg, 0.9% of the normal saline, 400 mg/kg, 600 mg/kg and 800 mg/kg burdock root extract BP1, respectively. The mortality, body weight and fasting blood glucose of each group were monitored weekly. After 9 weeks, the rats were dissected and blood biochemical parameters were measured. The pancreas of the kidney was paraffin-embedded and HE stained. In addition, part of renal tissue was used for fluorescent quantitative PCR. The results showed that the burdock root extract BP1 could restore the body weight of diabetic rats to a certain extent, regulate serum lipids and reduce the fasting blood glucose in diabetic rats. The electron microscopic observation showed that BP1 could significantly increase insulin secretion granules in pancreatic islet β cells. In addition, BP1 could promote the expression of IL-18 and TNF-α, and reduce the kidney injury in diabetic rats; the oxidative damage of kidney and pancreas in diabetic rats can be improved by increasing the activity of SOD enzyme. In conclusion, the burdock root extract BP1 had a protective effect on the kidney pancreas organ.